天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒

英文名：Aspartame

货号：K-ASPTM

规格：50 assays (manual) /

分析物意义： 食品中常见的增甜剂有阿斯巴甜、D-甘露醇、D-山梨醇和木糖醇

Megazyme检测试剂盒优点：K-ASPTM-方法新颖/只有试剂盒可用

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Aspartame (and breakdown products) in foodstuffs, beverages and other materials Principle: (pH 12.5) (1) Asp-Phe-O-Me → Asp-Phe + MeOH (dipeptidase M) (2) Asp-Phe + H₂O → L-aspartate + L-phenylalanine (glutamate-oxaloacetate transaminase) (3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate (L-malate dehydrogenase) (4) Oxaloacetate + NADH + H⁺→ L-malate + NAD⁺

Kit size: 50 assays (manual) / 500 (microplate) / 500 (auto-analyser)Method: Spectrophotometric at 340 nmReaction time: ~ 5 minDetection limit: 0.57 mg/LApplication examples: Soft drinks, artificial sweeteners, candies, mints, chewing gum, dieteticproducts, jam, chocolate and other materialsMethod recognition: Novel method

Advantages

• Very cost effective
• All reagents stable for > 12 months after preparation
• Only enzymatic kit available
• Measures aspartame and breakdown products (L-aspartate and aspartame acid)
• Very specific
• Very rapid reaction
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included
• Suitable for manual, microplate and auto-analyser formats

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