Beta葡聚糖[酵母和蘑菇]检测试剂盒 β-Glucan Assay Kit (Yeast & Mushroom)

产品名称：Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名称：Mushroom and yeast beta-glucan assay Kit

货号： K-YBGL

型号规格：100次

优点：成本低；所有试剂配制后的稳定性〉2年；只有酶试剂盒可用；；方法简单；包含标准品

检测原理：

检测方法：比色法

检测波长：510nm；

总检测时间：100min；

检测限：样品重量的1-100%；

适用样品：酵母制备、蘑菇制备及其他原料；

方法认证：新方法

For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit

Colourimetric method for the determination of Yeast and

Mushroom β-Glucan in yeast, mushroom, foodstuffs and

other materials

Principle:

(conc. HCl, 30°C, 45 min)

(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H₂O →

soluble glucan

(1.3 M HCl, 100°C, 2 h)

(2) Soluble glucan + H₂O → D-glucose + laminarisaccharides (trace)

(exo-1,3-β-glucanase + β-glucosidase)

(3) Laminarisaccharides + H₂O → D-glucose

(glucose oxidase)

(4) D-Glucose + H₂O + O₂→ D-gluconate + H₂O₂

(peroxidase)

(5) 2H₂O₂+ p-hydroxybenzoic acid + 4-aminoantipyrine →

quinoneimine + 4H₂O

(amyloglucosidase)

(6) α-Glucan + H₂O → D-glucose

Kit size: 100 assays

Method: Spectrophotometric at 510 nm

Total assay time: ~ 100 min

Detection limit: 1-100% of sample weight

Application examples:

Yeast preparations, mushroom preparations and other materials

Method recognition: Novel method

Advantages

• Very cost effective
• All reagents stable for > 12 months after preparation
• Only enzymatic kit available
• Simple format
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included