淀粉总量HK检测试剂盒

英文名： Total Starch HK Assay Kit

货号：K-TSHK

规格：100 assays per kit

A modification of AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for the measurement and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

UV-method for the determination of Total Starch in grains, animal feeds, foodstuffs and other materials Principle: (α-amylase, 100°C + DMSO) (1) Starch granules + H₂O → maltodextrins (amyloglucosidase) (2) Maltodextrins + H₂O → D-glucose (hexokinase) (3) D-Glucose + ATP → G-6-P + ADP (glucose-6-phosphate dehydrogenase) (4) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

Kit size: 100 assaysMethod: Spectrophotometric at 340 nmTotal assay time: ~ 90 minDetection limit: 1-100% of sample weightApplication examples: Cereal flours, food products and other materialsMethod recognition: AOAC (Method 996.11), AACC (Method 76-13.01), ICC (StandardMethod No. 168), and RACI (Standard Method)

Advantages

• Very competitive price (cost per test)
• All reagents stable for > 2 years after preparation
• Simple format
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included

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