SureCoat, Bovine Collagen, Solution, 3 mg/mL
货号:BM-5057-100ML
品牌: Advanced Biomatrix
报价:¥3200.00
商品描述
SureCoat® collagen is provided for coating surfaces and is not intended for gel applications.SureCoat® is highly purified (>99.9% collagen content) and has excellent coating functionality and properties.SureCoat® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
SureCoat® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.
SureCoat® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product.SureCoat™ is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
SureCoat® collagen is ideal for coating of surfaces and providing a thin layer for culturing cells.SureCoat® is not intended to be used for the formation of a gel.SureCoat® collagen is provided in user-friendly packaging for use and storage. This product is sterile filtered and is supplied as a ready to use solution.
Parameter, Testing, and Method |
SureCoat®Collagen Solution Catalog # 5057-100ML
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Sterilization Method
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Filtration
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Form
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Solution
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Package Size
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100 mL
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Storage Temperature
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2-10 °C
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Expiration Date
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Listed on product label and Certificate of Analysis
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Concentration (Biuret Protein Determination)
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2.9-3.2 mg/mL
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Purity (collagen concentration)
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> 99.9%
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pH
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Approx. pH 2, 0.01 N HCl
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Polyacrylamide Gel Electrophoresis (SDS – PAGE)
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>85% collagen containing within alpha, beta, and gamma bands<15% collagen contained with bands traveling faster than alpha
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Sterility
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No growth
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Endotoxin (LAL)
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<1.0 EU/ml
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Cell Attachment Assay
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Pass
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Source
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Bovine Hide – Pepsin Extracted
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Preparation and Usage
Coating Procedure
Note:Use these recommendations as guidelines todetermine the optimal coating conditions for your culturesystem.
- Transfer desired volume of collagen solution from the bottleto a dilution vessel as required. Dilute to desired concentrationusing sterile 0.01 N HCl solution. A typical workingconcentration may range from 10 to 100 g/ml.
- Add appropriate amount of diluted SureCoat® material tothe culture surface.
- Incubate at room temperature or 37°C, covered, for 1-2hours.
- After incubation, aspirate any remaining material.
- Rinse coated surfaces carefully with sterile medium or PBS,avoid scratching surfaces.
- Coated surfaces are ready for use. They may also be storedat 2-8°C damp or air dried if sterility is maintained.
Cell Attachment Bioassay
To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
Without UltraPure® |
With UltraPure® |
|
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Human Umbilical Vein Endothelial Cells (HUVEC) 10X |
|
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VERO Epithelial Cells 10X |
|
|
Human Dermal Fibroblast Cells 10X |
|
|
MDCK Epithelial Cells 10X |
References Downloads
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