Gtfa163 Glucansucrase (Reuteransucrase)
货号:P-GTFA163
规格:EA 100 Units
品牌:金山科研平台
报价:¥3455.00
商品描述
Enzyme activity: Gtfa163 Glucansucrase (Reuteransucrase) catalyses the transfer of glucose from sucrose to make a highly branched, high-molecular-weight α-D-glucan with α(1→4) glucosidic linkages but also some α(1→6)-linked glucosyl units and 4,6-disubstituted glucosyl units at the branching points.
As an example, glucose transferase activity was determined at 50°C for 60 hours in a 25 mM sodium acetate buffer at pH 4.7, containing 1 mM CaCl2 and 100 mM sucrose. This gave complete utilization of the sucrose with about 75% going into α-D-glucan.
Activity determination: Glucansucrase activity was determined at 50°C in a 25 mM sodium acetate buffer at pH 4.7, containing 10 mM CaCl2 and 30 nM enzyme.
Unit definition: One unit (U) of enzyme activity is the amount that leads to the release of 1 μmol of fructose from sucrose per minute.
Synonyms: glycoside hydrolase, glucosyltransferase, 4,6-alpha-Glucanotransferase, Reuteransucrase
Protein family: Glycosyl hydrolase family 70 ( GH70 ) – GH-H clan – CAZy database GH70 family
Enzyme classification: EC 2.4.1.-
Source: The lactic acid bacteria Lactobacillus reuteri strain 121
Protein sequence: NBCI protein entry
Structural information: The crystal structure of Gtfa163 Glucansucrase (Reuteransucrase) from Lactobacillus reuteri121 has been determined and shown here in Figure 1 (Pijning et al. 2012). – PDB entry 4AMC
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