内切木聚糖酶测定试剂盒（xylx6法）

英文名：endo-Xylanase Assay Kit (XylX6 Method)

货号：MK-XYLX6-1V

规格：100 assays (manual) / 200 assays (auto-analyser)

品牌： Megazyme

报价：￥3900.00

The XylX6 assay reagent for the measurement ofendo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-4⁵-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or otherexo-acting glycosidases on the XylX6 substrate. Incubation with anendo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by theendo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Colourimetric method for the determination ofendo-1,4-β-xylanaseinany sample type including crude enzyme extracts, fermentation broths or commercial enzyme preparations

Principle: (endo-1,4-β-xylanase) (1)3-Ketobutylidene-GX₅-β-PNP + H₂O → Blocked-GX_Y+ X_(5-Y)-β-PNP (β-glucosidase) (2) X_(5-Y)-β-PNP + H₂O → D-xylose + PNP (alkaline solution) (3) PNP → phenolate ion (yellow colour) Note:PNP = 4-nitrophenol

Kit size:K-XylX6-1V 100 assays (manual) / 200 (auto-analyser) orK-XylX6-2V 200 assays (manual) / 400 (auto-analyser)

Method: Spectrophotometric at 400/405nmTotal assay time: 10 minDetection limit: 5.3 x 10^-4 U/mLApplication examples: Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysisMethod recognition: Novel method

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