内切纤维素酶检测试剂盒(CELLG3方法)

英文名：Cellulase Assay Kit (CELLG3 Method)

货号：MK-CELLG3

规格：180 / 360 assays per kit / 720 (auto-analyser)

品牌： Megazyme

报价：￥6000.00

For the specific measurement of cellulase (endo-1,4-β-glucanase) in enzyme preparations and fermentation products.

Colourimetric method for the determination of endo-1,4-β- glucanase (cellulase) in enzyme preparations and fermentation products Principle: (endo-1,4-β-glucanase) (1) Benzylidene-G₃-β-CNP + H₂O → Blocked-G_X + G_(3-X)-β-CNP (thermostable β-glucosidase) (2) G_(3-X)-β-CNP + H₂O → D-glucose + CNP (alkaline solution) (3) CNP → phenolate ion (yellow colour) Note: CNP = 2-Chloro-4-nitrophenol

Kit size: 180 / 360 assays / 720 (auto-analyser)Method: Spectrophotometric at 400 nmTotal assay time: ~ 20 minDetection lim-it: 0.05 U/mLApplication examples: Fermentation broths, industrial enzyme preparations, biofuels researchMethod recognition: Novel methodNote: The analogous fluorimetric reagent (R-CELLF) is also available with 10-fold greater sensitivity

Advantages

• Very cost effective
• All reagents stable for > 2 years after preparation
• Completely specific for cellulase (endo-1,4-glucanase). The substrate is not hydrolysed by β-glucosidase, cellbiohyrolase or any other enzymes tested
• Kinetic assays possible due to significant phenolate ion presence (and UV absorbance) at pH 5-6
• Simple format. Well suited to automation
• Standard included

m.catalog.equl.cn.pdf:

Sample Pre-treatment Format: