GE Ficoll-Paque PLUS 淋巴细胞分离液是一种无菌、即用型密度培养 基,含有 Ficoll PM400、泛影、酸钠和乙er胺四乙酸 (EDTA ) 二钠钙。密度经过优化,可以从外周血中分离出人类淋巴细胞。
淋巴细胞分离液是一种根据细胞密度差异,借助离心产生的重力加速度,进行细胞的分离纯化的常用试剂 。常用的淋巴细胞分离液有Ficoll淋巴细胞分离液
淋巴细胞分离液的原理:
外周血中单个核细胞和单核细胞,其体积、形态和密度与其他细胞不同.淋巴细胞分离液是一种密度介于1.075∽1.090之间而近似于等渗的溶液,用它做密度梯度离心,使一定密度的细胞按相应密度梯度分布,从而将各种血细胞加以分离。
基本应用 :
可用于体外分离外周淋巴血细胞,也可以从其他来源中分离单核细胞,包括脐带血细胞和骨、髓细胞,适用于从血液及组织 匀浆中分离所需细胞,在医疗和医学生物中广泛应用。其他人及动物多种比重细胞分离液。
Parameter
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Ficoll-Paque PLUS
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Order Information
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For research use only.
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Application
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Cell isolation
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Density Max.
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1.078 g/ml
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Endotoxin Activity Max.
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< 0.12 EU/ml
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Properties
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Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.
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Estimated shelf life from manufacture date
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3 years
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Storage conditions
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4–30°C
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Stability
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Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate material in the solution
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Physical State
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Liquid
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pH Range
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5.5–7.5
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Color
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Colorless to slightly yellow
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Certificate of Analysis
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Yes
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Pack size
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6 x 100 mL/6X500 mL
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订购信息
货号 品牌
17144002 FICOLL PAQUE PLUS 6X100 ML
17144003 FICOLL PAQUE PLUS 6X500 ML
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