What's Included

• StemSpan™ SFEM II, 2 x 100 mL
• StemSpan™ Lymphoid Progenitor Expansion Supplement (10X), 5 mL
• StemSpan™ Lymphoid Differentiation Coating Material (100X), 2 x 0.25 mL
• StemSpan™ T Cell Progenitor Maturation Supplement (10X), 12.5 mL

Overview Use this two-step protocol to reproducibly expand thousands of CD4+CD8+double-positive (DP) T cells in cultures initiated with CD34+human cord blood (CB) cells. In step one, CD34+CB cells are cultured for 14 days in medium containing expansion supplement to promote their proliferation and differentiation into CD7+CD5+pro-T cells. In step two, pro-T cells generated during those first 14 days are cultured for another 4 weeks in medium containing maturation supplement to promote their maturation into DP cells. This medium kit is serum- and feeder-free, eliminating variation introduced by serum and stromal cell lines. For your convenience, all kit components are also available for purchase individually.StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion and differentiation of CD34+cells isolated from human CB samples to CD7+CD5+progenitor T (pro-T) cells. Additionally,StemSpan™ T Cell Progenitor Maturation Supplement (10X) enables further maturation of pro-T cells to DP cells. It also supports the maturation of DP cells to CD8 single-positive (SP) T cells with additional stimuli. StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) and StemSpan™ T Cell Progenitor Maturation Supplement (10X) are intended for use in combination withStemSpan™ SFEM II medium, and on plates coated withStemSpan™ Lymphoid Differentiation Coating Material (100X) . An optional protocol is available to further mature DP cells to CD8 SP T cells. This extended protocol uses a complete T cell progenitor maturation medium made with reagents included in the StemSpan™ T Cell Generation Kit and must be combined withImmunoCult™ CD3/CD28/CD2 T Cell Activator andIL-15 . SUBTYPE Specialized Media, Supplements CELL TYPE Hematopoietic Stem and Progenitor Cells, T Cells SPECIES Human APPLICATION Cell Culture, Differentiation, Expansion BRAND StemSpan AREA OF INTEREST Cancer, Cell Therapy Research, Immunology, Stem Cell Biology FORMULATION Serum-Free