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ClonaCell™ FLEX is a base methylcellulose medium that requires the addition of a 2X concentrate of the user’s own liquid medium to create a custom semi-solid cloning medium. This allows the user to adapt an existing liquid cloning method to a customized semi-solid cloning method. ClonaCell™ FLEX is recommended for selection and cloning of suspension-adapted mammalian cells, including CHO cells and hybridomas. The medium is chemically defined, animal component-free, and protein-free. It does not contain L-glutamine, selection agents, or phenol red. ClonaCell™ FLEX is compatible with a wide range of selection systems, including dihydrofolate reductase (DHFR), glutamine synthetase (GS), and hypoxanthine, aminopterin, thymidine (HAT). Benefits of semi-solid cloning: Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening. Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning. Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion. CONTAINS Methylcellulose Water SUBTYPE 专业培养基, 半固体培养基 CELL TYPE CHO细胞, HEK-293, Other, 杂交瘤 SPECIES 小鼠, 其他 APPLICATION 半固体克隆, 细胞培养 BRAND ClonaCell AREA OF INTEREST 抗体制备, 杂交瘤生成, 细胞系制备, 药物发现和毒性测试 FORMULATION 无动物源成分, 无血清, 化学成分确定, 无蛋白质