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biolog技术由美国的BIOLOG公司于1989年开发成功,进行95种*碳源的生化反应测试,zui初应用于纯种微生物鉴定,至今已经能够鉴定包括细菌,酵母菌和霉菌在内的2000多种病原微生物和环境微生物。1991年,Garland和Mill开始将这种方法应用于土壤微生物群落的研究。Biolog方法用于环境微生物群落研究,具有以下特点:1·灵敏度高,分辨力强。对多种SCSU的测定可以得到被测微生物群落的代谢特征指纹(metabolic finger-print),分辩微生物群落的微小变化。2·无需分离培养纯种微生物,可zui大限度地保留微生物群落原有的代谢特征。3·测定简便,数据的读取与记录可以由计算机辅助完成。微生物对不同碳源代谢能力的测定在一块微平板上一次完成,效率大大提高。
BIOLOG微平板由对照孔和95孔不同单一碳源孔组成,当接种纯培养的菌液时,其中一些孔的营养物质被利用,使孔中呈现出不同的颜色变化,从而构成该种微生物*的“代谢指纹 (MetabolicFingerprint),经相应的仪器记录,结果输入BIOLoG配套软件、与标准菌种的数据库进行比较,从而将被测菌种鉴定出来。
BIOLOG鉴定板与培养基 Microplate Panels and Media GEN II & GEN III MicroPlate Consumables | |
· Sold in increments of 10 plates/box · Consumable products are NON-RETURNABLE Catalog Number | Description | 1030 | GEN III MicroPlate (for both Gram-negative & Gram-positive bacteria) | 1011 | GN2 MicroPlate (GEN II for Gram-negative bacteria) | 1014 | GP2 MicroPlate (GEN II for Gram-positive bacteria) | 1007 | AN MicroPlate (GEN II for Anaerobic bacteria) | 1005 | YT MicroPlate (GEN II for Yeasts) | 1006 | FF MicroPlate (GEN II for Filamentous Fungi) |
Special Purpose MicroPlates · Sold in increments of 10 plates/box · Consumable products are NON-RETURNABLE Catalog Number | Description | 1013 | MT2 MicroPlate (for metabolic studies) | 1511 | SF-N2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-negative microbes) | 1514 | SF-P2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-positive microbes) | 1506 | ECO MicroPlate - 3 sets of the same 31 carbon sources in a 96 well Biolog MicroPlate. Used for characterization / community analysis) |
Biolog Prepared Plated Media · Sold in increments of 15 plates/pack Catalog Number | Description | 71102 | BUG (Biolog Universal Growth) Agar w/ 5% sheep blood | 71103 | BUG (Biolog Universal Growth) Agar w/ 0.25% maltose1 | 71005 | BUY (Biolog Universal Yeast) Agar1 |
Other Prepared Plated Media · Sold in increments of 10 plates/pack Catalog Number | Description | Bio-M1040 | Tryptic Soy Agar (TSA) | Bio-M1006 | Tryptic Soy Agar (TSA) w/ 5% sheep blood | Bio-M1012 | Chocolate Agar, enriched | 71007 | BUA (Biolog Universal Anaerobe) Agar w/5% sheep blood | Bio-M1065 | R2A Agar | 71106 | 2% Malt Extract Agar |
Biolog Dehydrated Media Catalog Number | Description | 70101 | BUG (Biolog Universal Growth) Agar - 500 g jar (approx. 435 plates) | 70007 | BUA (Biolog Universal Anaerobe) Agar | 70005 | BUY (Biolog Universal Yeast) Agar - 500 g jar (approx. 415 plates) |
GEN III Starter Kits · Kits containing the consumables a new user would need to begin using the OmniLog System for bacterial indentifications Catalog Number | Description | 39993A-O | 20 GEN III MicroPlates · 1 box of pipette tips (Ovation) · 1 CASE OF reservoirs · 1 package OF Inoculatorz (100 units individually packaged) · 20 tubes OF IF-A · 20 tubes OF IF-B | 39993A-M | 20 GEN III MicroPlates · 1 box of pipette tips (Matrix) · 1 CASE OF reservoirs · 1 package OF Inoculatorz (100 units individually packaged) · 20 tubes OF IF-A · 20 tubes OF IF-B | 39993B | 30 BUG+blood plated media for GEN III |
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