promega，M-MLV Reverse Transcriptase，M1701

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M-MLV Reverse Transcriptase · Protocol · MSDS · Components M-MLV Reverse Transcriptase · M-MLV Reverse Transcriptase · M-MLV RT 5X Buffer 10,000u 200u/μl M1701

QC TestsActivity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.SourceRecombinant E. coli strain.Storage Buffer20mM Tris-HCl （pH 7.5 at 25°C）, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.Storage ConditionsStore at –20°C.Unit DefinitionOne unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl （pH 8.3 at 25°C）, 7mM MgCl₂, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [³H]dTTP, 0.025mM oligo（dT）, 0.25mM poly（A） and 0.01% NP-40.For product intended use please see Patents & Disclaimers tab.