Genebridege 产品介绍

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我们是：代理商 金山科研平台咨询微信：jinshanbio Genebridege公司的FLPe表达质粒707-710可以促成大肠杆菌中以FLP为媒介的定点重组，在30°C时以低拷贝数传播。Flpe基因由λR启动子驱动，受控于不耐热cI857阻遏蛋白。温度从30°C上升至37°C会引发FLPe重组酶暂态表达。由于pSC101复制原点的缘故，表达质粒在37°C时不再复制，因此它们zui终会消失。与野生型FLP蛋白相比，FLPe拥有更好的耐热性，在37 – 40°C时显示出更强的重组酶活性产品信息如下：

AllRed/ETRecombination Kits come with tailored control experiments and harbor the same Red/ET proficiency plasmid.

Quick & Easy BAC Modification KitFor all types of BAC modifications.ID: K001690.00 €*DetailsManualOrder

Counter Selection BAC Modification KitFor advanced BAC modifications using a counter-selection cassette, also for bacterial chromosomes.ID: K002795.00 €*DetailsManualOrder

BAC Subcloning KitFor subcloning of DNA fragments from BACs and cosmids.ID: K003620.00 €*DetailsManualOrder

Quick & Easy Conditional Knock Out Kit - FRTFor fast and simple integration ofFRT-sitesinto large vector plasmids at any intended position （for generation of transgenic mouse models）.ID: K004690.00 €*DetailsManualOrder

Quick & Easy Conditional Knock Out Kit - loxPFor fast and simple integration ofloxP-sitesinto large vector plasmids at any intended position （for generation of transgenic mouse models）.ID: K005690.00 €*DetailsManualOrder

Quick & EasyE.coliGene Deletion KitFor fast knockouts or modifications of genes on theE.colichromosome.ID: K006620.00 €*DetailsManualOrderRed/ET Recombination Strains

E. colistrain GB05-dirDirect cloning-proficientE. colistrain for linear plus linear homologous recombinationID: K008590.00 €*DetailsManualOrder

E. colistrain GB08-redRecombineering-proficientE. colistrain for linear plus circular homologous recombinationID: K009590.00 €*DetailsManualOrderSelection Cassettes

Also available are accessory selection cassettes designed for use in Eukaryotes or Prokaryotes, some flanked by loxP- or FRT-sites. These sites will allow the subsequent removal of the selection marker by a Cre- or FLP-recombinase step if required. These cassettes may be very helpful for generating targeting constructs in mammals, or for introducing sequences without leaving a selection marker behind.

PGK-gb2-neoPro- and Eukaryotic Neomycin Selection CassetteID: A001290.00 €*DetailsManualOrder

FRT-PGK-gb2-neo-FRTPro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sitesID: A002290.00 €*DetailsManualOrder

loxP-PGK-gb2-neo-loxPPro- and Eukaryotic Neomycin Selection Cassette flanked by loxP-sitesID: A003290.00 €*DetailsManualOrder

FRT-PGK-gb2-neo-FRT-loxPPro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional downstream loxP siteID: A004290.00 €*DetailsManualOrder

loxP-FRT-PGK-gb2-neo-FRTPro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional upstream loxP siteID: A005290.00 €*DetailsManualOrder

FRT-gb2-cm-FRTProkaryotic Chloramphenicol Selection Cassette flanked by FRT-sitesID: A006290.00 €*DetailsManualOrder

loxP-gb2-cm-loxPProkaryotic Chloramphenicol Selection Cassette flanked by loxP-sitesID: A007290.00 €*DetailsManualOrder

FRT-gb2-amp-FRTProkaryotic Ampicillin Selection Cassette flanked by FRT-sitesID: A008290.00 €*DetailsManualOrder

loxP-gb2-amp-loxPProkaryotic Ampicillin Selection Cassette flanked by loxP-sitesID: A009290.00 €*DetailsManualOrder

FRT-PGK-gb2-hygro-FRTFRT flanked, Pro- and Eukaryotic Hygromycin Selection CassetteID: A010290.00 €*DetailsManualOrder

loxP-PGK-gb2-hygro-loxPloxP flanked, Pro- and Eukaryotic Hygromycin Selection CassetteID: A011290.00 €*DetailsManualOrder

iCre-FRT-neo-FRTCodon improved Cre （iCre） with attached FRT flanked, Pro- and Eukaryotic Neomycin Selection CassetteID: A012325.00 €*DetailsManualOrder

Expression Plasmids

The following recombinase expression plasmids are very useful to excise FRT- or loxP-flanked DNA stretches （such as selection markers） during a modification task. The plasmids are easily transformed into cells resulting in an activation of the recombinasesin vivo.

707-FLPe, tet

Flp Expression Plasmid with tetracycline resistance marker for use inE.colionlyID: A104290.00 €*DetailsManualOrder

708-FLPe, cm

Flp Expression Plasmid with chloramphenicol resistance marker for use inE.colionlyID: A105290.00 €*DetailsManualOrder

709-FLPe, ap

Flp Expression Plasmid with ampicillin resistance marker for use inE.colionlyID: A106275.00 €*DetailsManualOrder

710-FLPe, km

Flp Expression Plasmid with kanamycin resistance marker for use inE.colionlyID: A107275.00 €*DetailsManualOrder

705-Cre;cm

Cre Expression Plasmid with chloramphenicol resistance marker for use inE.colionlyID: A112290.00 €*DetailsManualOrder

706-Cre;tet

Cre Expression Plasmid with tetracycline resistance marker for use inE.colionlyID: A113290.00 €*DetailsManualOrder

pCAGGS-FLPe

Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Academia）.The use of the this product is governed by the terms and conditions of thepCAGGS-FLPe Material Transfer Agreement.ID: A201375.00 €*DetailsManualOrder

pCAGGS-FLPe

Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Industry）.The use of the this product is governed by the terms and conditions of thepCAGGS-FLPe Material Transfer Agreement.ID: A2023250.00 €*DetailsManualOrder