Merck蛋白抽提产品

BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法（如弗式压榨法和超声波法）的替代方法，这种试剂处理起来非常简便、快速，且成本低廉，适合制备表达蛋白用于下游的纯化等应用。

我们是：代理商 金山科研平台咨询微信：jinshanbio Merck蛋白抽提MERCK蛋白抽提1、BugBuster®蛋白抽提试剂——尤其适用于GST融合蛋白的抽提BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法（如弗式压榨法和超声波法）的替代方法，这种试剂处理起来非常简便、快速，且成本低廉，适合制备表达蛋白用于下游的纯化等应用。2、BugBuster Master Mix预混蛋白抽提试剂BugBuster Master Mix是将BugBuster蛋白抽提试剂，Benzonase核酸酶和rLysozyme™溶菌酶溶液按*化配比混合好的一种非常方便使用的蛋白抽提试剂，有助于在*化条件下获得可溶活性蛋白。

ProductSizeCat.No.Price（￥）BugBuster® Protein Extraction Reagent100 ml70584-3750.00500 ml70584-43,789.00BugBuster® Master Mix100 ml71456-31,700.00500 ml71456-45,200.00CytoBuster™ Protein Extraction Reagent50 ml71009-3824.00250 ml71009-43,122.00

Comparison ofE. colilysis methods50 ml samples of an induced 500 ml culture of BL21（DE3） containingpET-41a（+） encoding GST were harvested by centrifugation andresuspended in 2 ml 1X PBS, another commercially available proteinextraction reagent, or BugBuster Reagent. The sample in PBS wassonicated with 10 pulses at 50% duty for 30 s total. Samples in lysisreagent were treated according to their respective protocols. Extractswere clarified by centrifugation and assayed for GST enzymatic activity.

3、Benzonase® 核酸酶——赛多利斯病毒纯化试剂盒推荐使用应用范围

• ²蛋白抽提操作中降低样品粘度
• ²双向电泳中样品制备过程
• ²重组蛋白样品制备中去除核酸污染

ProductSizeCat.No.Price（￥）Benzonase® Nuclease, Purity > 90%10 ku70746-31,200.002.5 ku70746-4400.00

Viscosity reduction by Benzonase NucleaseE. coli BL21（DE3） cells containing a pET construct were suspended inBugBuster Reagent （5 ml/g wet weight）. Aliquots of the suspension weretreated with the indicated amounts of Benzonase Nuclease for 10 min at