5-Methylcytidine-5'-Triphosphate代理

5-甲基胞苷-5-三磷酸盐是TRILINK公司产品，用于干细胞研究用

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5-甲基胞苷-5-三磷酸盐是TRILINK公司新产品，用于干细胞研究用,5-methyl-ctp

Synonyms]5-methyl-dCTP5-methyldeoxycytidine triphosphateCPD-10945-methyl deoxycytidine-5'-triphosphate5-methyl-2'-deoxycytidine-5'-triphosphatecytidine 5'-（tetrahydrogen triphosphate）, 2'-deoxy-5-methyl-[[[5-[（4-amino-5-methyl-2-oxo-1H-pyrimidin-1-yl）]-3-hydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphinoyl]oxy-hydroxy-phosphinoyl]oxyphosphonic acid[Structure]

[ Properties Computed from Structure]

Molecular Weight481.183503 [g/mol]Molecular FormulaC₁₀H₁₈N₃O₁₃P₃XLogP-5.9H-Bond Donor6H-Bond Acceptor14Rotatable Bond Count8Tautomer Count3Exact Mass481.005247MonoIsotopic Mass481.005247Topological Polar Surface Area248Heavy Atom Count29Formal Charge0Complexity868Isotope Atom Count0Defined Atom StereoCenter Count0Undefined Atom StereoCenter Count3Defined Bond StereoCenter Count0Undefined Bond StereoCenter Count0Covalently-Bonded Unit Count1

Description5-Methyl-dCTP is widely used for construction of cDNA librariesIncorporation of 5-Methyl-dCTPM-MuLV Reverse TranscriptaseKlenow Fragment of DNA Polymerase ISequenase DNA Polymerase（Taq Polymerase, Vent） *Incorporation of Hg-dCTPDNA Polymerase IReferences to 5-Methyl-dCTPLefaucheuret al.（1998） Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs.J. Anim. Sci.76:1584.Nelsonet al.（1993） Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios.Nucl. Acids Res.21 （3）:681.Asamizuet al.（1999） A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags.DNA Research6:369.* Wonget al.（1991） PCR with 5-methyl-dCTP replacing dCTP.Nucl. Acids Res.19 （5）:1081.Reference to Hg-dCTPBanfalviet al.（1995） Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases.DNA Cell Biol.14 （5）:445.

N-1014-15-Methylcytidine-5'-TP1umole1317.5N-1014-105-Methylcytidine-5'-TP10umoles10625N-1014-55-Methylcytidine-5'-TP5umoles6205

Reference（s）Kariko K, Muramatsu H, Ludwig J, Weissman D. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. （2011） Nucleic Acids Research.Kormann M, Hasenpusch G, Aneja M, et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. （2011） Nature Biotechnology 29:154–157.Anderson, B., Muramatsu, H., Nallagatla, S.R., Bevilacqua, P.C., Sansing, L.H., Weissman, D. & Kariko, K. Incorporation of pseudouridine into mRNA enhances translation by dimishing PKR activation （2010） Nucleic Acids Research, 38（17）: 5884-5892.Warren et al., Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA, Cell Stem Cell （2010）, doi:10.1016/j.stem.2010.08.012.Kariko K, Muramatsu H, Welsh F, et al. Incorporation of Pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. （2008） Molecular Therapy （16）11: 1833-1840.Kariko, K., Buckstein, M., Ni, H. & Weissman, D. Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA （2005）. Immunity, 23（2）, 165-175.Lefmann M, et al. Novel Mass Spectrometry-based tool for genotypic identification of mycobacteria. （2004） Journal of Clinical Microbiology, 42（1）: 339-346.Hartmer R, Storm N, Boecker S, Rodi CP, Hillenkamp F, Jurinke C, van den Boom D. RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. （2003） Nucleic Acids Res., 31（9）: e47.Nguyen A, Zhao C, Dorris D, Mazumder A. Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios. （2002） BMC Biotechnology, 2（1）: 14.Van Rompay AR, Norda A, Linden K, Johansson M, Karlsson A. Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. （2001） Mol Pharmacol., 59（5）: 1181-6.