Merck Millipore代理AC113 Anti-Neomycin Phosphotransferase II Antibody | AC113;store at +2℃ to +8℃

2025-07-24

货号:AC113

品牌:Merck Millipore

规格:100UL

目录价:¥4740.00

市场价格:¥4029.00

会员价格:¥3792.00

金山科研平台,产品价格货期咨询微信:jinshanbio Description: Anti-Neomycin Phosphotransferase II Antibody | AC113 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Specificity: This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3'-phosphotransferase). View All» Molecular Weight: ~29 kDa observed. View All» Immunogen: Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II. View All» Background Information: Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. View All» Species Reactivity Note: Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment. View All» Control: Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-). View All» Quality Assurance: Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-). View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P00552 View All» Entrez Gene Number: YP_788126 View All» Gene Symbol:

  • neo

  • kan

  • nptII

    View All» Alternate Names:
    • Aminoglycoside 3'-phosphotransferase

    • APH(3')-II

    • APH(3')II

    • Kanamycin kinase, type II

    • Neomycin-kanamycin phosphotransferase type II

      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Product Name: Anti-Neomycin Phosphotransferase II View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»

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