货号:48-617MAG
品牌:Merck Millipore
规格:EA
目录价:¥11302.00
市场价格:¥9606.70
会员价格:¥9041.60
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Description:
MILLIPLEX MAP Human Stem Cell Pluripotency Magnetic Bead Panel 1, 48-617MAG
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Trade Name:
MILLIPLEX
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Qty/Pk:
96-well plate
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Product Overview:
The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Panel 1 (Cat. No. 48-617MAG) is a premixed panel to be used for the simultaneous quantification of the following 4 analytes in cell lysates: c-Myc, Nanog, Oct 3/4, and Sox2. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.
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Background Information:
The expression of the transcription factors Oct 3/4, Sox2 and Nanog play a critical role in the maintenance of pluripotency and self renewal in stem cells. c-Myc, a member of the helix-loop-helix-leucine zipper family, is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell differentiation and cell proliferation. In stem cells it seems to control the balance between stem cell self-renewal and differentiation. Thus the presence of the four proteins Oct-3/4, Sox2, Nanog and c-Myc are indicators for the pluripotency of iPS cells and human embryonic stem cells.
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Key Applications:
Multiplexing
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Application Notes:
o This is an overnight (4°C) incubation assay.o This kit must be run using the provided Assay Buffer.o This assay requires 25 μL diluted filtered cell lysate per well.o The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL).
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Species Reactivity:
Human
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
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Product Name:
MILLIPLEX MAP Human Stem Cell Pluripotency Magnetic Bead Panel 1, 48-617MAG
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Specificity:
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within this panel.
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Analytes Available:
Nanog, Oct 3/4, Sox2, c-Myc
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Configuration:
Premixed
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Panel Type:
MAGNETIC Stem Cell Characterization
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Detection Methods:
Luminex xMAP
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Assay Panel:
Everything you need in a single kit.
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