货号：17-10269

品牌：Merck Millipore

规格：25assays

目录价：￥6357.00

市场价格：￥5403.45

会员价格：￥5085.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+™ Phospho-Histone H3 (Ser28) Antibody | 17-10269 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+™ Phospho-Histone H3 (Ser28) set includes the Phospho-Histone H3 (Ser28) antibody, a Normal Rabbit IgG, and control primers which amplify a 166 bp region of the human GAPDH promoter. The Phospho-Histone H3 (Ser28) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Phospho-Histone H3 (Ser28)-associated chromatin. View All» Molecular Weight: ~17 kDa observed View All» Epitope: Phosphorylated Ser28 View All» Immunogen: KLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser28. View All» Modifications: Phosphorylation View All» Background Information: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. View All» Application Notes: Chromatin Immunoprecipitation: Representative lot data.Sonicated chromatin prepared from untreated or colcemid-treated HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 5 µl Normal rabbit IgG, or 5 µl Anti-phospho-Histone H3 (Ser28) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of phospho-Histone H3 (Ser28)- associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH promoter region for untreated and treated chromatin samples (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Western Blot Analysis: Representative lot data.HeLa acid extract (lane 1) and recombinant Histone H3 (lane 2) were probed with Anti-phospho-Histone H3 (Ser28) (1:2,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. (Figure 3)Arrow indicates phospho-Histone H3 (Ser28) (~17 kDa).Dot Blot Specificity Analysis:Representative lot data.Histone peptides with various modifications (see table) were probed with Anti-phospho-Histone H3 (Ser28). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. (Figure 4)Immunocytochemistry AnalysisRepresentative lot dataConfocal fluorescent analysis of HeLa cells using Anti-phospho-Histone H3 (Ser28) (Red). Actin filaments have been labeled with AlexaFluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitotic cell nucleus. (Figure 5)Peptide Inhibition Assay: A 1:500 dilution from a representative lot was blocked by phospho-Histone H3 (Ser28) peptides, but not non-specific peptides. View All» Control: Includes normal rabbit IgG and primers specific for human GAPDH promoter View All» Quality Assurance: Chromatin Immunoprecipitation:Representative lot data.Sonicated chromatin prepared from colcemid-treated HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 5 µl Normal rabbit IgG, or 5 µl Anti-phospho-Histone H3 (Ser28) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of phospho-Histone H3 (Ser28)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH promoter region (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Purification Method: Affinity purified View All» Presentation: Anti- Phospho-Histone H3 (Ser28) (Rabbit Polyclonal). One vial containing 125 μl of purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C.Normal Rabbit IgG. Two vials containing 75 μL of normal rabbit IgG in buffer containing 0.01M PBS with 0.1% sodium azide. Store at -20°C.ChIP Primers, human GAPDH promoter. One vial containing 75 μL of 5 μM each primer specific for human GAPDH. Store at -20°C.FOR: TAC TAG CGG TTT TAC GGG CGREV: TCG AAC AGG AGG AGC AGA GAG CGA View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q16695 View All» Entrez Gene Number: NP_003484.1 View All» Gene Symbol:
  • HIST3H3

  • H3FT
    View All» Alternate Names:
    • Histone H3.1t

    • H3/t

    • H3t

    • H3/g
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Dot Blot

      • Peptide Inhibition Assay
        View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. View All» UniProt Summary: FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus. Chromosome. View All» Product Name: ChIPAb+™ Phospho-Histone H3 (Ser28) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Affinity Purified View All» Host: Rabbit View All» Packaging: 25 assays per set. Recommended use: 5 μL of antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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