货号：17-10062

品牌：Merck Millipore

规格：25 assays

目录价：￥4960.00

市场价格：￥4216.00

会员价格：￥3968.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ E2F-3 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ E2F-3 set includes the E2F-3 antibody, a negative control normal mouse IgG, and qPCR primers which amplify a 100 bp region of human CDC2 promoter. The E2F-3 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of E2F-3-associated chromatin. View All» Specificity: E2F-3 View All» Molecular Weight: ~58 kDa View All» Epitope: Unknown View All» Immunogen: Recombinant full-length human E2F-3 protein View All» Isotype: IgG2a View All» Background Information: E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human and mouse. Weak reactivity with rat. View All» Application Notes: Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Mouse IgG, or 1 µg Anti-E2F-3 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of E2F-3 associated DNA fragments was verified by qPCR using ChIP Primers, human CDC2 promoter as a positive locus, and a intergenic region near human CALML5 as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Western Blot Analysis: Representative lot data.Lysates from HeLa cells probed with anti-E2F-3 at 2 μg/mL. Arrow indicates E2F-3 (~58 kDa) (Figure 3). View All» Control: Includes normal mouse IgG and primers specific for human CDC2 coding region. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Mouse IgG, or 1 µg Anti-E2F-3 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of E2F-3 associated DNA fragments was verified by qPCR using ChIP Primers, human CDC2 promoter (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Purification Method: Protein A purfied View All» Presentation: Anti-E2F-3 (mouse monoclonal), . One vial containing 25 ug of protein A purified mouse IgG2a in ~180 uL of storage buffer (10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before addition of 30% glycerol). Store at -20°C.Normal Mouse IgG. One vial containing 25 ug purified IgG in 25 uL storage buffer containing 0.1% sodium azide. Store at -20°C.ChIP Primers, human CDC2 coding region, . One vial containing 75 μL of 5 μM each primer specific for human CDC2 coding region. Store at -20°C. FOR: CGC CCT TTC CTC TTT CTT TCREV: ATC GGG TAG CCC GTA GAC TT View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O00716 View All» Entrez Gene Number: NP_001940.1 View All» Gene Symbol:
  • E2F3

  • KIAA0075

  • E2F-3

  • MGC104598

  • DKFZp686C18211
    View All» Alternate Names: Transcription factor E2F3 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation

    • Immunocytochemistry
      View All» Entrez Gene Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. View All» UniProt Summary: FUNCTION: SwissProt: O00716 # Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.SIZE: 465 amino acids; 49162 Da SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.SUBCELLULAR LOCATION: Nucleus.SIMILARITY: SwissProt: O00716 ## Belongs to the E2F/DP family. View All» Brand Family: Upstate View All» Product Name: ChIPAb+ E2F-3 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Purified View All» Host: Mouse View All» Packaging: 25 assays per set. Recommended use: 1 μg of antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：http://16ao.com/thread-147429.htm
      © Merck Millipore代理17-10062 ChIPAb+ E2F-3, clone PG37，产品报价联系微信jinshanbio