货号：06-933

品牌：Merck Millipore

规格：100UL

目录价：￥6742.00

市场价格：￥5730.70

会员价格：￥5393.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-acetyl-Lysine Antibody | 06-933 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Acetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA. View All» Molecular Weight: Dependent upon the molecular weight of the lysine acetylated protein being detected. View All» Immunogen: A mixture of chemically acetylated antigens. View All» Modifications: Acetylation View All» Isotype: IgG View All» Background Information: In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. View All» Species Reactivity: Vertebrates View All» Species Reactivity Note: Broad species reactivity expected. View All» Application Notes: Immunoprecipitation: 5 μL of a previous lot immunoprecipitated in vitro acetylated recombinant p300.Western Blot Analysis: A 1:1000 dilution of the previous lot of the antibody detected:• Acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5 μM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B).• GST-p53 (100 ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C). View All» Control: TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue. View All» Quality Assurance: Evaluated by western blot on acid-extracted proteins from sodium butyrate treated HeLa cells, chemically acetylated BSA, acetylated histones and to a lesser degree, other proteins in trichostatin A treated Cos-1 cell lysate.Western Blot Analysis: 1:1000-1:2000 dilution of this antibody detected:• Acetylated histones in acid-extracted proteins from sodium butyrate (5 mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A).• Chemically acetylated BSA (10 ng) in a dot blot assay. View All» Purification Method: Antiserum View All» Presentation: Rabbit IgG serum containing 0.05% sodium azide and 30% glycerol. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Western Blotting

• Immunoprecipitation
  View All» Brand Family: Upstate View All» Product Name: Anti-acetyl-Lysine Antibody | 06-933 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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