货号：MABE328

品牌：Merck Millipore

规格：100UL

目录价：￥5359.00

市场价格：￥4555.15

会员价格：￥4287.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MeCP2 Antibody, clone 4H7 | Cat. No. MABE328 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the C-terminus of MeCP2, which has been epitope mapped between amino acids 310-492. View All» Molecular Weight: ~75 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~55 kDa. The calculated molecular weight is 53 kDa, however MeCP2 has been observed between ~75-100 kDa in western blots (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). View All» Epitope: C-terminus View All» Immunogen: Full length, strep-tagged N-terminus of rat MeCP2. View All» Clone: 4H7 View All» Isotype: IgG2aκ View All» Background Information: DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA. It represses transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families. View All» Species Reactivity:

• Mouse

• Porcine

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Mouse. Predicted to react with Porcine and Rat based on 100% sequence homology. View All» Application Notes: Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse whole brain cell lysates (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). View All» Control: Mouse brain tissue nuclear extract View All» Quality Assurance: Evaluated by Western Blot in mouse brain tissue nuclear extract.Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract. View All» Purification Method: Protein G purified View All» Presentation: Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q00566 View All» Entrez Gene Number: NP_073164 View All» Gene Symbol: Mecp2 View All» Alternate Names:
  • Methyl-CpG-binding protein 2

  • MeCp-2 protein

  • MeCp2
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation

    • Chromatin Immunoprecipitation (ChIP)

    • Immunocytochemistry

    • Immunohistochemistry
      View All» Product Name: Anti-MeCP2, clone 4H7 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rat View All»
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