货号：MAB5308

品牌：Merck Millipore

规格：100Ug

目录价：￥6588.00

市场价格：￥5599.80

会员价格：￥5270.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-BACE Antibody, C-terminus, clone 61-3E7 | MAB5308 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with BACE (beta-site APP Cleaving Enzyme). Shows no reactivity to BACE2 by Western blot. View All» Molecular Weight: ~ 60-75 kDa View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to the C-terminus of human BACE. View All» Clone: 61-3E7 View All» Isotype: IgG1 View All» Background Information: Alzheimer's disease (AD) is characterized by the progressive formation in the brain of insoluble amyloid plaques and vascular deposits consisting of the 4-kD amyloid b-peptide (Ab). Ab generation is initiated by proteolytic cleavage of the amyloid precursor protein (APP) at the N-terminal of Ab by b-secretase. The Ab peptide is then released by proteolytic cleavage at its C-terminus by g-secretase. Because both these proteases are prime candidates for therapeutic intervention, an intense search has been underway to identify these two enzymes.A human transmembrane aspartic-protease (Asp2), referred to as BACE, has been characterized and shown to have all the properties of b-secretase. Four groups in all have now confirmed that BACE (or Asp2) is a convincing candidate for b-secretase. BACE is an N-glycosylated integral membrane aspartyl protease with Mr=70 kDa. Mature BACE is produced from the immature form through a series of post-translational proteolytic cleavages and glycosylation. Sequence analysis has revealed that the immature form of BACE contains an N-terminal signal sequence (residues 1-21) followed by a large catalytic domain, a single transmembrane domain (residues 461-477), and a short cytoplasmic domain (residues 478-501). The signal sequence (1-21) is cleaved from the immature form by a signal peptidase located in the endoplasmic reticulum (ER), yielding the proBACE protein (Mr=75 kDa) which starts at residue 22. The proBACE protein is modified by cleavage of 24 N-terminal residues (aa 22-45), producing the mature BACE protein. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Primate
  View All» Application Notes: Western blotting: 1 μg/mL; recognizes pro and mature forms: ~60-75kDa on reducing westerns. BACE is N-terminally glycosylated which causes the wide size range. Immunohistochemistry on paraformaldehyde fixed tissues from human, rat, mouse and monkey. Immunocytochemistry on cells expressing BACE. Acetone or methanol fixation preferred; 4% PFA 5', RT followed by 0.1% triton X-100 1 hour, can also be used. 1:200-1:500 is recommended, optimization is necessary. Immunoprecipitation: Optimal working dilutions must be determined by end user. View All» Control: Brain View All» Purification Method: Protein A purfied View All» Presentation: Purified immunoglobulin. Liquid. Buffer = 0.02M Sodium Phosphate, 0.25M NaCl with 0.1% sodium azide. View All» Storage Conditions: Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: P56817 View All» Entrez Gene Number:
  • NM_012104.3

  • NM_138971.2

  • NM_138972.2

  • NM_138973.2
    View All» Gene Symbol:
    • BACE1

    • ASP2

    • beta-secretase

    • KIAA1149

    • Memapsin-2

    • BACE

    • memapsin-2

    • HSPC104

    • FLJ90568

    • EC 3.4.23.46
      View All» Alternate Names: ASP2; BACE1; Beta Secretase; beta-Site APP Cleaving Enzyme View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P56817 # Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.SIZE: 501 amino acids; 55764 Da SUBUNIT: Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4.SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.TISSUE SPECIFICITY: Brain.SIMILARITY: SwissProt: P56817 ## Belongs to the peptidase A1 family. View All» Brand Family: Chemicon View All» Product Name: Anti-BACE Antibody, C-terminus, clone 61-3E7 | MAB5308 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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