货号：04-207

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Chk1 (N-term) Antibody, clone E250, Rabbit | 04-207 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes Chk1 at the N-Terminus. View All» Molecular Weight: 54 kDa View All» Epitope: N-Terminus View All» Immunogen: Synthetic peptide directed against the N-terminus of human Chk1. View All» Clone: E250 View All» Isotype: IgG View All» Background Information: DNA damage activates ATM, which phosphorylates and activates CHK1 and CHK2/Cds1. The principal substrates of CHK1 and CHK2 are cdc25C (inhibited by phosphorylation), p53 (stabilized by phosphorylation), and BRCA1 (scatters through the nucleus following phosphorylation). Activation of p53 results in cell cycle arrest, or induction of apoptosis. Cdc25C inhibition prevents dephosphorylation of the inhibitory phosphotyrosine residue on cdc2/cdk1, thus preventing the G2/M transition. BRCA1 dispersion through the nucleus is associated with DNA repair. View All» Species Reactivity:

• Human

• Rat
  View All» Species Reactivity Note: Proven to react with human and mouse. Not expected to react with rat. View All» Application Notes: Immunocytochemistry: A previous lot of this antibody was used on Immunocytochemistry View All» Control: K562 whole cell lysate. View All» Quality Assurance: Routinely evaluated by Western blot on K562 whole cell lysate.Western Blot Analysis:A 1:1000 dilution of this lot was used to detect Chk1 K562 whole cell lysate View All» Purification Method: Unpurified View All» Presentation: Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O14757 View All» Entrez Gene Number: NM_001114121.1 View All» Gene Symbol:
  • CHK1

  • EC 2.7.11.1
    View All» Alternate Names:
    • CHK1 (checkpoint, S.pombe) homolog

    • CHK1 checkpoint homolog

    • CHK1 checkpoint homolog (S. pombe)

    • Checkpoint, S. pombe, homolog of, 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» UniProt Summary: FUNCTION:Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. Ref.1 Ref.6 Ref.9 Ref.13 Ref.15 Ref.16 Ref.17 Ref.18 Ref.20 Ref.22 Ref.24 Ref.25 Ref.27 Ref.29 Ref.33 Ref.35 Ref.36Catalytic activity ATP + a protein = ADP + a phosphoprotein.SUBUNIT: Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta. Ref.1 Ref.35 Ref.14 Ref.21 Ref.23 Ref.30 Ref.32 Ref.34SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note= Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B. Ref.1 Ref.29 Ref.14 Ref.21 Ref.31SPECIFICITY: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. Ref.1DOMAIN: The autoinhibitory region (AIR) inhibits the activity of the kinase domain. Ref.28PTM: Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity. Ref.1 Ref.9 Ref.13 Ref.16 Ref.17 Ref.20 Ref.27 Ref.36 Ref.21 Ref.30 Ref.32 Ref.28 Ref.8 Ref.10 Ref.19 Ref.26 Ref.38 Ref.39 Ref.40Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion By similarity.SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-Chk1 (N-term), clone E250, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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