货号：MAB1004

品牌：Merck Millipore

规格：250Ug

目录价：￥5820.00

市场价格：￥4947.00

会员价格：￥4656.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Interleukin-6 Antibody, clone 4B6 | MAB1004 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with ovine IL-6. No reactivity detected to recombinant ovine IL-1beta, IL-8, MCP or TNFalpha. SPECIES REACTIVITY: The antibody is likely to react with caprine (goat) IL-6. View All» Immunogen: Recombinant ovine IL-6 View All» Clone: 4B6 View All» Isotype: IgG1 View All» Species Reactivity: Sheep View All» Application Notes: Capture antibody in ELISA. Recommended coating concentration 5 μg/mL. See suggested protocol. Optimal working dilutions must be determined by the end user. Suggested ELISA Protocol For Ovine IL-6 1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-6 antibody (Chemicon Catalog Number MAB1004) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC. 2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST). 3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST. 4. Dilute rabbit anti-IL-6 (Chemicon Catalog Number AB1839) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST. 5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST. 6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid. Notes: The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates. Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents. Assay sensitivity may be altered by adjusting incubation times and reagent concentrations. View All» Presentation: Liquid in PBS containing 0.1% sodium azide. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P05231 View All» Entrez Gene Number: NM_000600.2 View All» Gene Symbol:

• IL6

• IL-6

• HSF

• BSF2

• BSF-2

• CDF

• IFNB2

• HGF
  View All» Alternate Names: IL-6 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: ELISA View All» UniProt Summary: FUNCTION: SwissProt: P05231 # IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.SIZE: 212 amino acids; 23718 Da SUBCELLULAR LOCATION: Secreted.PTM: N- and O-glycosylated.DISEASE: SwissProt: P05231 # Genetic variations in IL6 are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement. & At least 1 polymorphism in the IL6 gene renders HIV- infected men susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of spindle cells mixed with angiomatous tissue. A relatively rare malignant skin tumor that results in multifocal purplish coloured papules or plaques that eventually form nodules. Most commonly seen in patients who suffer from AIDS.SIMILARITY: SwissProt: P05231 ## Belongs to the IL-6 superfamily. View All» Brand Family: Chemicon View All» Product Name: Anti-Interleukin-6 Antibody, clone 4B6 | MAB1004 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 250 µg View All» Format: Purified View All» Host: Mouse View All»
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