货号：700238

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥4692.00

市场价格：￥4222.80

会员价格：￥4222.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 ABfinity™ Recombinant Rabbit Monoclonal Antibody validated to Human and expected reactivity with human, mouse, rat, equine, opossum, bovine, and zebrafish. This antibody is validated for use in Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry. 4E-BP1 [pT37] is encoded by the 1978 gene, also known as Eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, EIF4EBP1.Eukaryotic initiation factor 4E binding protein 1 (4E-BP1), also known as PHAS, is a ~20 kDa member of a family of eIF4E-binding proteins whose binding affinity to eIF4E is regulated by its phosphorylation. It inhibits cap-dependent translation by binding to eIF4E on the same site that overlaps the binding site for eIF4G, preventing its binding to the latter and eventually leading to an increase in mRNA translation. The phosphorylatin of 4E-BP1 is critical in determining cell fate by controlling translation initiation and apoptotic potency. 4E-BP1 is hyperphosphorylated in response to several external stimuli including hormones, growth factors, mitogens, cytokines and G-protein–coupled receptors and in response to stress conditions including nutrient deprivation. The phosphorylation of 4E-BP1 increases in response to activated phosphoinositol 3’-kinase (PI-3K) or its downstream effector Akt⁄PKB. 4E-BP1 is believed to mediate PI-3K and FRAP⁄mTOR signaling and is phosphorylated on at least six serine and threonine sites (Thr 37, Thr 46, Ser 65, Thr 70, Ser 83, and Ser 112). The phosphorylation of these sites is believed to occur in an orderly fashion where phosphorylation of threonine 37 and 46 by FRAP⁄mTOR is a priming step for subsequent phosphorylation of 4E-BP1 at the carboxy-terminal sites.Gingras, A.C., et al. (1999) Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism. Genes Dev. 13(11):1422-1437. Gingras, A.C., et al. (2001) Hierarchical phosphorylation of the translation inhibitor 4E-BP1.Genes Dev. 15(21):2852-2864. Li, S., et al. (2002) Translational control of cell fate: availability of phosphorylation sites on translational repressor 4E-BP1 governs its proapoptotic potency. Mol. Cell Biol. 22(8):2853-2861. Li, W. and B.E. Sumpio (2005) Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. Am. J. Physiol. Heart Circ. Physiol. 288(4):H1591-H1597. Wang, X., et al. (2005) Distinct signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on initiation factor 4E-binding proteins. Mol. Cell Biol. 25(7):2558-2572. Greenberg, V.L. and S.G. Zimmer (2005) Paclitaxel induces the phosphorylation of the eukaryotic translation initiation factor 4E-binding protein 1 through a Cdk1-dependent mechanism. Oncogene 24(30):4851-4860. Zhou, L., et al. (2005) 4E-binding protein phosphorylation and eukaryotic initiation factor-4E release are required for airway smooth muscle hypertrophy. Am. J. Respir. Cell Mol. Biol. 33(2):195-202. Stephens, L., et al. (2005) Phosphoinositide 3-kinases as drug targets in cancer. Curr. Opin. Pharmacol. 5(4):357-365. Armengol G., et al (2007) 4E-binding protein 1: a key molecular "funnel factor" in human cancer with clinical implications. Cancer Res. 67(16):7551-7555. 规格

Reactivity:	Human
Validated Application:	Flow Cytometry,Immunohistochemistry,Immunofluorescence,Western Blot
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Monoclonal
Clone ID:	52H37L2
Antibody Fragment:	Whole Antibody
Target Gene:	4E-BP1 [pT37]
Gene ID:	1978
Accession Number:	Q13541
Label or Dye:	Unconjugated
Target Modification State:	Phospho
Purification:	Affinity Purified
Concentration:	0.5 mg⁄ml
Color:	Unconjugated
Form:	Liquid
Neutralizing Antibody:	No
Platform:	Fluorescence Microscope,Confocal Microscope
Product Size:	100 µg
Quantity:	1
Volume:	200 µl
Shipping Condition:	Wet Ice

内容及储存 2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.