货号：4461105

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：EACH

目录价：￥15335.00

市场价格：￥13801.50

会员价格：￥13801.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 TaqMan® OpenArray® MicroRNA Panels provide many of the advantages of TaqMan® MicroRNA Assays in a high-throughput plate and are ideal for medium to large miRNA profiling studies. TaqMan® OpenArray® MicroRNA Panels enable the running of hundreds of TaqMan® MicroRNA Assays in parallel per sample. This greatly simplifies the generation of a comprehensive miRNA profile for up to 36 samples in a single working day.Key product features: • Comprehensive coverage-generate miRNA profiles across 750 rodent (mouse and rat) well-characterized miRNAs with as little as 100 nanograms of total RNA• High throughput-run up to 36 samples per 8-hour working day with minimal hands-on time and no need for robotics• Cost effective-3 samples can be run per panel in 33 nanoliter reaction volumes resulting in significant reagent cost savings Provides Comprehensive Coverage of Well-Studied miRNAs in Sanger miRBaseMany well-characterized rodent (mouse and rat) miRNA sequences from the Sanger miRBase are included. The content is derived from miRBase v15, providing close to full coverage of Sanger well-studied miRNAs using highly-validated TaqMan® MicroRNA Assays.Rodent

Megaplex™ Primer Pools	MicroRNA Targets	Control (+ve)	Control (-ve)
A	375	5	1
B	375	5	1
Total	750

Streamlined Workflow and High Throughput Sample HandlingThe workflow is completed in five steps (Fig 1):1. Split total RNA sample and process in parallel using Megaplex™ Primer Pools A and B and the supporting TaqMan® MicroRNA Reverse Transcription Kit.2. Resulting cDNA is preamplified prior to real-time PCR analysis using Megaplex™ PreAmp Pools and TaqMan® PreAmp Master Mix.3. Preamplified cDNA is diluted, mixed 1:1 with TaqMan® OpenArray® Real-Time PCR Master Mix in the 384-well OpenArray® Sample Loading Plate.4. TaqMan® OpenArray® Rodent MicroRNA Panels are automatically loaded using the AccuFill™ System.5. Up to 3 panels are cycled simultaneously on an OpenArray® Real-Time PCR Instrument.High Concordance to Single-tube AssaysThe TaqMan® OpenArray® Rodent MicroRNA Panel has demonstrated high concordance with an R2 value of 0.97 to the mostly commonly used tool for validation: individual TaqMan MicroRNA Assays (Fig 2). The same panel is used in the TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex, enabling accurate answers to help minimize validation.Additional Required Reagents These products are also required to run the TaqMan® OpenArray® Rodent MicroRNA Panel:• Megaplex™ Primer Pools (PN 4444750 or 4444766)• TaqMan® MicroRNA Reverse Transcription Kit (PN 4366596 or 4366597)• TaqMan® PreAmp Master Mix (PN 4391128)• TaqMan® OpenArray® Real-Time PCR Master Mix (PN 4462159 or 4462164)• OpenArray® Real-Time Accessories Kit (PN 4453975)• OpenArray® 384-well Sample Plates (PN 4406947)• OpenArray® AccuFill™ System Tips (PN 4457246 or 4458107)Download OpenArray® Plate FilesOpenArray® Plate Files (TPF) are needed to process your TaqMan® OpenArray® MicroRNA Panels on the OpenArray® Real-Time PCR System.For Research Use Only. Not for use in diagnostics procedures. 规格

Form:	Dried Down
Format:	OpenArray® Plate
Species:	Mouse,Rat
Platform:	OpenArray®
Product Size:	1 panel
Reaction Speed:	Standard
Research Category:	Epigenetics,Gene Regulation
Number of Reactions:	3072 Reactions

内容及储存 Store at or below –20°C. Do not store in a frost-free freezer.