货号：552299

品牌：BD Pharmingen

规格：500ug

目录价：￥8484.00

市场价格：￥5344.92

会员价格：￥4666.20

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Purified immunoglobulin in 10 mM phosphate buffer, pH 7.2 with 150 mM NaCl (0.2 mm filtered). No sodium azide. Endotoxin level is 0.01 ng/mg of protein.

Preparation and Storage

Preparation: The antibody was purified from tissue culture supernatant by affinity chromatography.Storage: The antibody solution should be stored undiluted at 4°C.

Usage

This antibody has been tested by LAL assay for endotoxin level and by immunofluorescent staining ( 1 mg/million cells) with flow cytometric analysis to assure specificity and reactivity. Since this antigen is expressed at low density on the cell surface, it may be desirable to use a second-step reagent conjugated to a “bright” fluorochrome, such as PE-conjugated goat anti-rat Ig (Cat. no. 554052). Other reported applications include immunoprecipitation¹ and blocking assays.^1,2,3 Since applications vary, each investigator must determine dilutions appropriate for individual use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

552299

Brand

BD Pharmingen™

Name

CD51

Alias

Integrin α_V chain

Concentration

1.0 mg/ml

Size

0.5mg

RegulatoryStatus

RUO

Clone

RMV-7

Format

NA/LE

Isotype

IgG₁, κ

W.B. Positive Control

none

Immunogen

Mouse (BALB/c) IL-2-activated killer (LAK) Cells

Strain Host

SD

Reactivity

QC Testing : Mouse

Applications

Blocking	- Reported
Flow cytometry	- Routinely Tested
Immunoprecipitation	- Reported

Clone - References

1. Takahashi, K., T. Nakamura, M. Koyanagi, K. Kato, Y. Hashimoto, H. Yagita, and K. Okumura. 1990. A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction. J. Immunol. 145: 4371 – 4379.2. Narumiya, S., Y. Abe, Y. Kita, K. Miyake, K. Nakajima, T.X. Watanabe, Y. Oka, H. Sugiyama, H. Yagita, K. Okumura, T. Hamaoka, and H. Fujiwara. 1994. Pre-B cells adhere to fibronectin via interactions of integrin a5/av with RGDS as well as of integrin a4 with two distinct V region sequences at its different binding sites. Int. Immunol. 6: 139 – 147.3. In Mouse Genome Database (MGD), Mouse Genome Informatics, The Jackson Laboratory, Bar Harbor, Maine. World Wide Web (URL: http//www.informatics.jax.org/searches/mlc.cgi?10502). February 2000.4. Takahashi, K., T. Nakamura, H. Adachi, H. Yagita, and K. Okumura. 1991. Antigen-independent T cell activation mediated by a very late activation antigen-like extracellular matrix receptor. Eur. J. Immunol. 21: 1559 – 1562.