货号：550797

品牌：BD Pharmingen

规格：100ug

目录价：￥1525.00

市场价格：￥960.75

会员价格：￥838.75

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Purified immunoglobulin in 10 mM phosphate buffer, pH 7.2 with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

Preparation: The antibody was purified from tissue culture supernatant by affinity chromatographyStorage: The antibody solution should be stored undiluted at 4°C

Usage

This antibody has been tested by immunofluorescent staining ( 1 µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Other reported applications include immunoprecipitation,¹ inhibition of T-cell–mediated cytolysis,¹ complement-mediated cytotoxicity,¹ and immunohistochemical staining (IHC) of acetone-fixed frozen and zinc-fixed paraffin-embedded sections.²¹ IHC of formalin-fixed paraffin-embedded sections is not recommended. Since applications vary, each investigator must determine dilutions appropriate for individual use.

Caution

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

550797

Brand

BD Pharmingen™

Name

CD8b

Alias

Ly-3

Concentration

0.5 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

H35-17.2

Format

Purified

Isotype

IgG_2b, κ

W.B. Positive Control

none

Immunogen

5-day MLR, C57BL/6 anti-BALB/c

Reactivity

QC Testing : Mouse

Applications

Cytotoxicity	- Reported
Flow cytometry	- Routinely Tested
Immunohistochemistry-frozen	- Tested During Development
Immunohistochemistry-paraffin	- Not Recommended
Immunohistochemistry-zinc-fixed	- Tested During Development
Immunoprecipitation	- Reported
Inhibition	- Reported