货号：550595

品牌：BD Pharmingen

规格：100ug

目录价：￥5434.00

市场价格：￥3423.42

会员价格：￥2988.70

金山科研平台，产品价格货期咨询微信：jinshanbio Contents

with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

The antibody was purified from tissue culture supernatant by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions. The solution is free of unconjugated antibody. The conjugate should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Usage

This antibody conjugate has been tested by immunofluorescent staining ( 1 mg/million cells) with flow cytometric analysis to assure specificity and reactivity. PE has been reported to bind mouse B cells via FcgII receptors. For immunostaining of mouse cells, this binding can be prevented by preincubating cells with Mouse Fc Block™ (Cat. no. 553141/553142).⁷ Please contact BD PharMingen`s Technical Service Department for more information. Since applications vary, each investigator must determine dilutions appropriate for individual use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

550595

Brand

BD Pharmingen™

Name

Ly-49I

Concentration

0.2 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

YLI-90

Format

PE

Isotype

IgG₁, κ

W.B. Positive Control

none

Immunogen

CHO-K1 cells transfected with the B6 allele of the Ly-49I gene, K1ra9

Strain Host

BALB/c

Reactivity

QC Testing : Mouse

Applications

Flow cytometry

- Routinely Tested

Clone - References

1. Coles, M.C., C.W. McMahon, H. Takizawa, and D.H. Raulet. 2000. Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors. Eur. J. Immunol. 30: 236 – 244.2. In Mouse Genome Database (MGD), Mouse Genome Informatics, The Jackson Laboratory, Bar Harbor, Maine. World Wide Web (URL: http://www.informatics.jax.org/). 07/2000.3. BD PharMingen. Unpublished results.4. Takei, F., J. Brennan, and D.L. Mager. 1997. The Ly-49 family: genes, proteins and recognition of class I MHC. Immunol. Rev. 155: 67 – 77.5. Lian, R.H., Y. Li, S. Kubota, D.L. Mager, and F. Takei. 1999. Recognition of class I MHC by NK receptor Ly-49C: identification of critical residues. J. Immunol. 162: 7271 – 7276.6. George, T., Y.Y.L. Yu, J. Liu, C. Davenport, S. Lemieux, E. Stoneman, P.A. Mathew, V. Kumar, and M. Bennett. 1997. Allorecognition by murine natural killer cells: lysis of T-lymphoblasts and rejection of bone-marrow grafts. Immunol. Rev. 155: 29 – 40.