货号：550532

品牌：BD Pharmingen

规格：500ug

目录价：￥11508.00

市场价格：￥7250.04

会员价格：￥6329.40

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with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

The antibody was purified from tissue culture supernatant by affinity chromatography. The antibody solution should be stored undiluted at 4°C.

Usage

This antibody has been tested by immunofluorescent staining ( 1 mg/million cells) with flow cytometric analysis to assure specificity and reactivity. Because of the low density expression of 4-1BBL on activated B lymphocytes, we recommend the use of a bright second-step reagent, such as PE-conjugated anti-rat Ig polyclonal antibody (Cat. no. 559970). Other reported applications include the blocking of the functional interaction of 4-1BBL with 4-1BB.¹ Since applications vary, each investigator must determine dilutions appropriate for individual use.

Caution

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

550532

Brand

BD Pharmingen™

Name

4-1BB Ligand

Concentration

0.5 mg/ml

Size

0.5mg

RegulatoryStatus

RUO

Clone

TKS-1

Format

Purified

Isotype

IgG_2a, κ

Immunogen

BALB/c mouse B lymphoma line 2PK-3

Strain Host

SD

Reactivity

QC Testing : Mouse

Applications

Blocking	- Reported
Flow cytometry	- Routinely Tested

Clone - References

1. Akiba, H., Y. Miyahira, M. Atsuta, K. Takeda, C. Nohara, T. Futagawa, H. Matsuda, T. Aoki, H. Yagita, and K. Okumura. 2000. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental Leishmaniasis. J. Exp. Med. 191: 375 – 380.2. Pollok, K.E., S.H. Kim, and B.S. Kwon. 1995. Regulation of 4-1BB expression by cell-cell interactions and the cytokines, interleukin-2 and interleukin-4. Eur. J. Immunol. 25: 488 – 494.3. DeBenedette, M.A., A. Shahinian, T.W. Mak, and T.H. Watts. 1997. Costimulation of CD28^- T lymphocytes by 4-1BB ligand. J. Immunol. 158: 551 – 559.4. Pollok, K.E., Y.-J. Kim, Z. Zhou, J. Hurtado, K.K. Kim, R.T. Pickard, and B.S. Kwon. 1993. Inducible T cell antigen 4-1BB. Analysis of expression and function. J. Immunol. 150: 771 – 781.5. Pollok, K.E., Y.-J. Kim, J. Hurtado, Z. Zhou, K.K. Kim, and B.S. Kwon. 1994. 4-1BB T-cell antigen binds to mature B cells and macrophages, and costimulates anti-m-primed splenic B cells. Eur. J. Immunol. 24: 367 – 374.6. BD PharMingen. Unpublished results.7. Goodwin, R.G., W.S. Din, T. Davis-Smith, D.M. Anderson, S.D. Gimpel, T.A. Sato, C.R. Maliszewski, C.I. Brannan, N.G. Copeland, N.A. Jenkins, T. Farrah, R.J. Armitage, W.C. Fanslow, and C.A. Smith. 1993. Molecular cloning of a ligand for the inducible T cell gene 4-1BB: a member of an emerging family of cytokines with homology to tumor necrosis factor. Eur. J. Immunol. 23: 2631 – 2641.

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