货号：550514

品牌：BD Pharmingen

规格：1EA

目录价：￥5233.00

市场价格：￥3296.79

会员价格：￥2878.15

金山科研平台，产品价格货期咨询微信：jinshanbio Contents

Purified antibody in 10 mM sodium phosphate, pH 7.2, with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

Preparation: For the generation of the MABTNFR1-B1 hybridoma, BALB/c mice were immunized with purified, full-length human TNFRI protein that was expressed by insect cells that were infected with a recombinant human TNFRI-baculovirus expression vector. MABTNFR1-B1 specifically binds to natural and recombinant truncated forms of TNFRI, including the human TNFRStorage: The antibody solution should be stored undiluted at 4°C.

Caution

Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Conditions

The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. PharMingen will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Catalog Number

550514

Brand

BD Pharmingen™

Name

CD120a

Alias

TNF Receptor Type I

Concentration

0.5 mg/ml

Size

0.5mg

RegulatoryStatus

RUO

Clone

MABTNFR1-B1

Format

Purified

Isotype

IgG_2a, κ

W.B. Positive Control

none

Reactivity

QC Testing : Human

Applications

Flow cytometry

- Routinely Tested

Clone - References

1. Gehr, G., R. Gentz, M. Brockhaus, H. Loetscher, and W. Lesslauer. 1992. Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation. J. Immunol. 149:911-917.2. Hohmann, H. P., R. Remy, M. Brockhaus, and A. P. van Loon. 1989. Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha). J. Biol. Chem. 264:14927-14934.3. Brockhaus, M., H. J. Schoenfeld, E. J. Schlaeger, W. Hunziker, W. Lesslauer, and H. Loetscher. 1990. Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 87:3127-3131.4. Aggarwal, S., S. Gollapudi, and S. Gupta. 1999. Increased TNF-alpha-induced apoptosis in lymphocytes from aged humans: changes in TNF-alpha receptor expression and activation of caspases. J. Immunol. 162:2154-2161.5. Erikstein, B. K., E. B. Smeland, H. K. Blomhoff, S. Funderud, K. Prydz, W. Lesslauer, and T. Espevik. 1991. Independent regulation of 55-kDa and 75-kDa tumor necrosis factor receptors during activation of human peripheral blood B lymphocytes. Eur. J. Immunol. 21:1033-1037.6. Heilig, B., M. Mapara, M. Brockhaus, K. Krauth, and B. Dorken. 1991. Two types of TNF receptors are expressed on human normal and malignant B lymphocytes. Clin Immunol Immunopathol 61:260-267.7. Munker, R., J. DiPersio, and H. P. Koeffler. 1987. Tumor necrosis factor: receptors on hematopoietic cells. Blood 70:1730-1734.8. Zola, H. 1995. Detection of cytokine receptors by flow cytometry. In Current Protocols in Immunology. J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, and W. Strober, eds. Greene Publishing Associates and Wiley-Interscience, New York, p. 6.21.1-6.21.18.9. Zola, H. 1994. Detection of receptors for cytokines and growth factors. The Immunologist 2:47-50.10. Wallach, D., H. Engelmann, Y. Nophar, D. Aderka, O. Kemper, V. Hornik, H. Holtmann, and C. Brakebusch. 1991. Soluble and cell surface receptors for tumor necrosis factor. Agents Actions Suppl. 35:51-57.11. Browning, J. L., I. Dougas, A. Ngam-ek, P. R. Bourdon, B. N. Ehrenfels, K. Miatkowski, M. Zafari, A. M. Yampaglia, P. Lawton, W. Meier, C. P. Benjamin, and C. Hession. 1995. Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors. J. Immunol. 154:33-46.

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