货号：550320

品牌：BD Pharmingen

规格：100ug

目录价：￥3800.00

市场价格：￥2394.00

会员价格：￥2090.00

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with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

The antibody was purified from tissue culture supernatant by affinity chromatography. The antibody solution should be stored undiluted at 4°C.

Usage

This antibody has been tested by immunofluorescent staining ( 1 µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Since this antigen is expressed at low density on lymphoid cell surfaces, it may be desirable to amplify staining by using a biotinylated second-step antibody, such as goat anti-rat Ig polyclonal antibody (Cat. no. 554050), followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. no. 554061) or Streptavidin-APC (Cat. no. 554067). In order to obtain a bright staining on LAK (Lymphokine-activated killer) cells, we suggested the use of A-LAK cells (adherent-LAK cells) that had been activated with 3,000 U/ml of recombinant human IL-2 (Cat. no. 554603). Other reported applications include western blot analysis² and blocking of IL-2/IL-15–activated NK-cell cytotoxicity against mouse fibrosarcoma L929 target cells.¹ Immunohistochemical staining of frozen or paraffin-embedded sections is not recommended. Since applications vary, each investigator must determine dilutions appropriate for individual use.

Caution

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Conditions

BD PharMingen will not be responsible for violations or patent infringements which may occur with the use of our products.

Catalog Number

550320

Brand

BD Pharmingen™

Name

CD253

Alias

TRAIL

Concentration

0.5 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

N2B2

Format

Purified

Isotype

IgG_2a, κ

W.B. Positive Control

none

Immunogen

Mouse Trail-transfected 2PK-3 cells

Strain Host

F344

Reactivity

QC Testing : Mouse

Applications

Flow cytometry	- Routinely Tested
Western Blot	- Reported

Clone - References

1. Kayagaki, N., N. Yamaguchi, M. Nakayama, K. Takeda, H. Akiba, H. Tsutsui, H. Okamura, K. Nakanishi, K. Okumura, and H. Yagita. 1999. Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J. Immunol. 163: 1906 - 1913.2. BD PharMingen. Unpublished results.3. Degli-Esposti, M. 1999. To die or not to die--the quest of the TRAIL receptors. J. Leukoc. Biol. 65: 535 - 542.4. Wu, G.S., T.F. Burns, Y. Zhan, E.S. Alnemri, and W.S. El-Deiry. 1999. Molecular cloning and functional analysis of the mouse homologue of the KILLER/DR5 tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor. Cancer Res. 59: 2770 - 2775.5. Mariani, S.M., and P.H. Krammer. 1998. Surface expression of TRAIL/Apo-2 ligand in activated mouse T and B cells. Eur. J. Immunol. 28: 1492 - 1498.6. Mariani, S.M., and P.H. Krammer. 1998. Differential regulation of TRAIL and CD95 ligand in transformed cells of the T and B lymphocyte lineage. Eur. J. Immunol. 28: 973 - 982.7. Musgrave, B.L., T. Phu, J.J. Butler, A.P. Makrigiannis, and D.W. Hoskin. 1999. Murine TRAIL (TNF-related apoptosis inducing ligand) expression induced by T cell activation is blocked by rapamycin, cyclosporin A, and inhibitors of phosphatidylinositol 3-kinase, protein kinase C, and protein tyrosine kinases: evidence for TRAIL induction via the T cell receptor signaling pathway. Exp. Cell Res. 252: 96 - 103.