货号：550078

品牌：BD Pharmingen

规格：1EA

目录价：￥4789.00

市场价格：￥3017.07

会员价格：￥2633.95

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R-PE-conjugated immunoglobulin in 10 mM sodium phosphate, pH 7.2, with 150 mM NaCl and 0.09% (w/v) sodium azide.

Preparation and Storage

Preparation: The D21-1351 antibody was purified from tissue culture supernatant via Protein G affinity chromatography. The antibody was conjugated with R-phycoerythrin (PE) under optimal conditions. The solution is free of unconjugated antibody. Storage: The conjugate should be stored undiluted at 4°C and protected from prolonged exposure to light.

Caution

Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Conditions

The information disclosed herein is not to be construed as a recommendation to use the above product in violation of any patents. PharMingen will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Catalog Number

550078

Brand

BD Pharmingen™

Name

MIP-1β

Concentration

0.2 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

D21-1351

Format

PE

Isotype

IgG₁

W.B. Positive Control

none

Immunogen

Recombinant Human MIP-1β

Reactivity

QC Testing : Human

Applications

Flow cytometry

- Routinely Tested

Clone - References

1. Lipes, M. A., M. Napolitano, K. T. Jeang, N. T. Chang, and W. J. Leonard. 1988. Identification, cloning, and characterization of an immune activation gene. Proc. Natl. Acad. Sci. USA 85:9704-8.2. Sherry, B., P. Tekamp-Olson, C. Gallegos, D. Bauer, G. Davatelis, S. D. Wolpe, F. Masiarz, D. Coit, and A. Cerami. 1988. Resolution of the two components of macrophage inflammatory protein 1, and cloning and characterization of one of those components, macrophage inflammatory protein 1b. J. Exp. Med. 168:2251-9.3. Ziegler, S. F., T. W. Tough, T. L. Franklin, R. J. Armitage, and M. R. Alderson. 1991. Induction of macrophage inflammatory protein-1 beta gene expression in human monocytes by lipopolysaccharide and IL-7. J. Immunol. 147:2234-9.4. Napolitano, M., K. B. Seamon, and W. J. Leonard. 1990. Identification of cell surface receptors for the Act-2 cytokine. J. Exp. Med. 172:285-9.5. Combadiere, C., S. K. Ahuja, H. L. Tiffany, and P. M. Murphy. 1996. Cloning and functional expression of CC CKR5, a human monocyte CC chemokine receptor selective for MIP-1a, MIP-1b, and RANTES. J. Leukoc. Biol. 60:147-52.6. Raport, C. J., J. Gosling, V. L. Schweickart, P. W. Gray, and I. F. Charo. 1996. Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1b, and MIP-1a. J. Biol. Chem. 271:17161-6.7. Samson, M., O. Labbe, C. Mollereau, G. Vassart, and M. Parmentier. 1996. Molecular cloning and functional expression of a new human CC-chemokine receptor gene. Biochemistry 35:3362-7.8. Bernardini, G., J. Hedrick, S. Sozzani, W. Luini, G. Spinetti, M. Weiss, S. Menon, A. Zlotnik, A. Mantovani, A. Santoni, and M. Napolitano. 1998. Identification of the CC chemokines TARC and macrophage inflammatory protein-1 beta as novel functional ligands for the CCR8 receptor. Eur. J. Immunol. 28:582-8.9. Prussin, C., and D. Metcalfe. 1995. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J. Immunol. Meth. 188:117-28.

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