ScienCellGK010人类黑素细胞发育和色素沉着

2023-06-29

GK010 ScienCell 人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

金山科研平台作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK010 ScienCell人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit,产品简介:

产品品牌:ScienCell

产品货号:GK010

产品名称:GK010 ScienCell人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

金山科研平台是ScienCell中国区总代理,提供各种基因表达分析相关品,ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK010 ScienCell人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit, 产品说明:

参与黑色素细胞发育和色素沉着途径的关键基因的基因表达谱。该试剂盒根据数据库和文献研究选择了40个基因。

GeneQuery™ PCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增目标基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

gene expression profiling of key genes involved in melanocyte development and pigmentation pathways. 40 genes are selected in this kit based on database and literature research.

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ 人类黑色素细胞发育和色素沉着 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ADCY3

CTNNB1

GRB2

MC1R

PRKACA

β-actin

ADCY3

CTNNB1

GRB2

MC1R

PRKACA

β-actin

B

AKT1

EDNRB

GSK3B

MITF

QPCT

GAPDH

AKT1

EDNRB

GSK3B

MITF

QPCT

GAPDH

C

AKT3

EP300

HRAS

MLANA

RPS6KA1

LDHA

AKT3

EP300

HRAS

MLANA

RPS6KA1

LDHA

D

BCL2

FOXD3

KIT

OCA2

SOS1

NONO

BCL2

FOXD3

KIT

OCA2

SOS1

NONO

E

BRAF

FZD3

KITLG

PAX3

SOX10

PPIH

BRAF

FZD3

KITLG

PAX3

SOX10

PPIH

F

CDH1

FZD4

LEF1

PIK3CA

TRP2

GDC

CDH1

FZD4

LEF1

PIK3CA

TRP2

GDC

G

CREB1

GNB2L1

MAPK1

PMEL

TYR

PPC

CREB1

GNB2L1

MAPK1

PMEL

TYR

PPC

H

CREBBP

GPR143

MAPK3

POMC

TYRP1

NTC

CREBBP

GPR143

MAPK3

POMC

TYRP1

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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