ScienCellGK026人类Wnt信号通路qPCR检测试剂盒

2023-06-29

GK026 ScienCell 人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK026 ScienCell人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit,产品简介:

产品品牌; ScienCell

产品货号:GK026

产品名称:GK026 ScienCell人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK026 ScienCell人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™ Human Wnt信号通路qPCR阵列试剂盒(GQH-WNT)旨在促进Wnt信号转导中涉及的88个关键基因的基因表达谱分析。 Wnt信号通路以其在癌变和胚胎发育中的作用而闻名。 该试剂盒侧重于规范的Wnt信号。 如何根据目的对所包含的基因进行分组的简要示例如下所示:

配体:所有已知的WNTs。

受体: FZD1-10, SMO, LRP5/6, RSPO1-4, LGR4-6

受体换能器: DVL1, DVL2, DVL3, CSNK1E, MARK1

转录因子:CTNNB1,TCF7L1,TCF7L2,LEF1,PYGO1

监管要素:NLK、NKD1、AES、SFRP1、LRP1

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Wnt Signaling Pathway qPCR Array Kit (GQH-WNT) is designed to facilitate gene expression profiling of 88 key genes involved in Wnt signaling transduction. The Wnt signaling pathway is well known for its role in carcinogenesis and embryonic development. This kit focuses on canonical Wnt signaling. Brief examples of how included genes may be grouped according to purpose are shown below:

· Ligands: all known WNTs

· Receptors: FZD1-10, SMO, LRP5/6, RSPO1-4, LGR4-6

· Receptor transducers: DVL1, DVL2, DVL3, CSNK1E, MARK1

· Transcription factors: CTNNB1, TCF7L1, TCF7L2, LEF1, PYGO1

· Regulatory elements: NLK, NKD1, AES, SFRP1, LRP1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人类 Wnt 信号通路 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

AES

CSNK2A1

DKKL1

FZD2

GSK3A

LRP1

PORCN

RSPO4

SMO

WNT16

WNT6

β-actin

B

APC

CTNNB1

DVL1

FZD3

GSK3B

LRP5

PPP2R4

RYK

TCF7L1

WNT2

WNT7A

GAPDH

C

AXIN1

CTNNBIP1

DVL2

FZD4

HNF1A

LRP6

PYGO1

SFRP1

TCF7L2

WNT2B

WNT7B

LDHA

D

AXIN2

CUL1

DVL3

FZD5

KREMEN1

MARK1

ROR1

SFRP2

TLE1

WNT3

WNT8A

NONO

E

BCL9

DKK1

FBXW7

FZD6

LEF1

MARK2

ROR2

SFRP4

WNT1

WNT3A

WNT8B

PPIH

F

CDC37

DKK2

FRZB

FZD7

LGR4

MARK3

RSPO1

SKP1

WNT10A

WNT4

WNT9A

GDC

G

CSNK1A1L

DKK3

FZD1

FZD8

LGR5

NKD1

RSPO2

SKP2

WNT10B

WNT5A

WNT9B

PPC

H

CSNK1E

DKK4

FZD10

FZD9

LGR6

NLK

RSPO3

SMARCA4

WNT11

WNT5B

ZNRF3

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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