GK035 ScienCell 人体缺氧反应qPCR检测试剂盒,Human Hypoxia Response qPCR Array Kit

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理，咨询微信：jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京诺博莱德作为ScienCell品牌的代理商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK035 ScienCell人体缺氧反应qPCR检测试剂盒，Human Hypoxia Response qPCR Array Kit，产品简介：

产品品牌: ScienCell

产品货号：GK035

产品名称：GK035 ScienCell人体缺氧反应qPCR检测试剂盒，Human Hypoxia Response qPCR Array Kit

产品规格：One 96-well plate, one 20ul reaction per well

GK035 ScienCell人体缺氧反应qPCR检测试剂盒，Human Hypoxia Response qPCR Array Kit， 产品说明：

缺氧是指与正常有氧条件相比氧气含量降低的任何环境。 当细胞缺氧时，会诱导缺氧反应，长时间缺氧通常会导致局部细胞环境的生理补偿，例如缺氧区域的血管生成增加。 ScienCell 的 GeneQuery™ 人类缺氧反应 qPCR 阵列试剂盒 （GQH-HPX） 旨在促进参与细胞对低氧条件反应的 88 个关键基因的基因表达谱分析。 如何根据反应类型对所包含的基因进行分组的简要示例如下所示：

细胞代谢： ENO1， HK1， PFKFB3， PGK1， SLC2A1

炎症： CXCL8， IL1A， CCL2， IL6， THBD

血管松弛：AHSP，PTGIS

细胞死亡或增殖：BNIP3L、NOS3、PIM1、EPO

血管生成： KDR， PDGFB， PLAU， TEK， FLT1

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有说明，否则引物集可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

Hypoxia refers to any environment with reduced oxygen content compared to normal aerobic conditions. The hypoxic response is induced when cells are oxygen deprived and prolonged deprivation often results in physiological compensation by the local cellular environment, such as increased angiogenesis to the hypoxic region. ScienCell's GeneQuery™ Human Hypoxia Response qPCR Array Kit (GQH-HPX) is designed to facilitate gene expression profiling of 88 key genes involved in the cellular response to low-oxygen conditions. Brief examples of how included genes may be grouped according to response type are shown below:

· Cellular metabolism: ENO1, HK1, PFKFB3, PGK1, SLC2A1

· Inflammation: CXCL8, IL1A, CCL2, IL6, THBD

· Vasorelaxation: AHSP, PTGIS

· Cell death or proliferation: BNIP3L, NOS3, PIM1, EPO

· Angiogenesis: KDR, PDGFB, PLAU, TEK, FLT1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人内皮细胞生物学 qPCR 阵列板布局*（8 个粗体和斜体对照）

提示：基因选择可能会根据新的研究和开发进行更新

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