ScienCellGK039人类先天免疫反应qPCR检测试剂盒

2023-06-29

GK039 ScienCell 人类先天免疫反应qPCR检测试剂盒,Human Innate Immune Response qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK039 ScienCell人类先天免疫反应qPCR检测试剂盒,Human Innate Immune Response qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK039

产品名称:GK039 ScienCell人类先天免疫反应qPCR检测试剂盒,Human Innate Immune Response qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK039 ScienCell人类先天免疫反应qPCR检测试剂盒,Human Innate Immune Response qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人类先天免疫反应qPCR阵列试剂盒(GQH-IIR)有助于人类先天免疫系统中关键基因的基因表达谱分析。 先天免疫反应在微生物感染或异物出现时被激活,包括补体系统和适应性免疫系统的激活。 一个单独的阵列可用于分析适应性免疫反应。 如何根据功能对所包含的基因进行分组的简要示例如下所示:

细菌反应: TLR1, TLR5, NOD1, LY96, TRAM1

病毒反应: TLR3, DDX58, IFIH1, MYD88, IRAK4

自我识别: TLR2, TLR4, TLR7, TLR8, CLEC4E

炎症小体成分:CASP1,CASP5,PYCARD,NLRP1

补体系统成分:C3,MBL2,MASP1,CFB,CD46

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Innate Immune Response qPCR Array Kit (GQH-IIR) facilitates gene expression profiling of key genes in the human innate immune system. The innate immune response is activated upon microbial infection or foreign substance presentation and includes activation of the complement system and adaptive immune systems. A separate array is available for analyzing the adaptive immune response. Brief examples of how included genes may be grouped according to function are shown below:

· Bacteria response: TLR1, TLR5, NOD1, LY96, TRAM1

· Virus response: TLR3, DDX58, IFIH1, MYD88, IRAK4

· Self recognition: TLR2, TLR4, TLR7, TLR8, CLEC4E

· Inflammasome component: CASP1, CASP5, PYCARD, NLRP1

· Complement system component: C3, MBL2, MASP1, CFB, CD46

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人类先天免疫应答 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ATF3

CASP5

CLEC6A

IFIH1

IRAK1

LYZ

NAP1L1

OTUD5

TICAM1

TLR5

TRAF6

β-actin

B

C3

CD46

CLEC7A

IFNA1

IRAK2

MAP3K7

NFAM1

PYCARD

TICAM2

TLR6

TRAM1

GAPDH

C

C5

CFB

DDX58

IFNB1

IRAK3

MAPK8

NFATC1

RIPK2

TIRAP

TLR7

TRAM2

LDHA

D

C6

CFD

DHX58

IFNG

IRAK4

MASP1

NFKB1

RNF125

TLR1

TLR8

TRIM25

NONO

E

C7

CFH

EYA4

IL18

IRF3

MASP2

NFKBIE

SYK

TLR10

TLR9

TYROBP

PPIH

F

C8A

CFP

FADD

IL1A

IRF7

MAVS

NLRP1

TAB2

TLR2

TNF

UBE2N

GDC

G

CARD9

CHUK

HCST

IL1B

JUN

MBL2

NOD1

TAB3

TLR3

TRADD

UBE2V1

PPC

H

CASP1

CLEC4E

HMGB1

IL6

LY96

MYD88

NOD2

TBK1

TLR4

TRAF3

UNC93B1

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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