GK060 ScienCell 人类昼夜节律qPCR检测试剂盒,Human Circadian Rhythm qPCR Array Kit

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理，咨询微信：jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京诺博莱德作为ScienCell品牌的代理商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK060 ScienCell人类昼夜节律qPCR检测试剂盒，Human Circadian Rhythm qPCR Array Kit，产品简介：

产品品牌: ScienCell

产品货号：GK060

产品名称：GK060 ScienCell人类昼夜节律qPCR检测试剂盒，Human Circadian Rhythm qPCR Array Kit

产品规格：One 96-well plate, one 20ul reaction per well

GK060 ScienCell人类昼夜节律qPCR检测试剂盒，Human Circadian Rhythm qPCR Array Kit， 产品说明：

ScienCell的GeneQuery™人类昼夜节律qPCR阵列试剂盒（GQH-CIR）分析了88个关键基因，这些基因参与了大约24小时周期内发生的调节变化。 昼夜节律主要是对光变化的反应，存在于大多数生物体中。 不要与生物钟混淆，昼夜节律会影响睡眠模式、激素释放、体温和其他稳态功能。 以下是如何根据其功能对所包含的基因进行分组的简要示例：

时钟调节： ARNTL， BHLHE40， CLOCK， PER1， RORA

酪蛋白激酶：CSNK1A1、CSNK1D、CSNK1E、CSNK2A1

CREB途径：CAMK2A，HTR7，MAPK1，PRKACB，AANAT

转录因子：SMAD4，IRF1，NFIL3，RORC，SP1，

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有说明，否则引物集可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Circadian Rhythm qPCR Array Kit (GQH-CIR) profiles 88 key genes involved in the regulating changes that occur on a roughly 24-hr cycle. Circadian rhythms are primarily responses to changes in light and are found in most living organisms. Not to be confused with biological clocks, circadian rhythms can influence sleep patterns, hormone release, body temperature, and other homeostatic functions. Below are brief examples of how included genes may be grouped according to their function:

· Clock regulation: ARNTL, BHLHE40, CLOCK, PER1, RORA

· Casein kinases: CSNK1A1, CSNK1D, CSNK1E, CSNK2A1

· CREB pathway: CAMK2A, HTR7, MAPK1, PRKACB, AANAT

· Transcription factors: SMAD4, IRF1, NFIL3, RORC, SP1,

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人类昼夜节律 qPCR 阵列板布局*（8 个粗体和斜体对照）

提示：基因选择可能会根据新的研究和开发进行更新

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