ScienCellGK072人类适应性免疫反应qPCR检测试剂盒

2023-06-29

GK072 ScienCell 人类适应性免疫反应qPCR检测试剂盒,Human Adaptive Immune Response qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

金山科研平台作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK072 ScienCell人类适应性免疫反应qPCR检测试剂盒,Human Adaptive Immune Response qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK072

产品名称:GK072 ScienCell人类适应性免疫反应qPCR检测试剂盒,Human Adaptive Immune Response qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK072 ScienCell人类适应性免疫反应qPCR检测试剂盒,Human Adaptive Immune Response qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人类适应性免疫反应qPCR阵列(GQH-AIR)有助于对参与适应性免疫或获得性免疫的88个关键基因进行基因表达分析。 脊椎动物有两个主要的免疫系统:先天性和适应性。 虽然先天免疫系统通常负责宿主防御病原体,但适应性免疫系统负责防御特定病原体,从而赋予免疫记忆。 如何根据其在适应性免疫反应中的功能对所包含的基因进行分组的简要示例如下所示:

抗原呈递:CANX、HLA-A、HLA-DPB1、PSMB8、TAP2

Th1标志物:IL12RB1,CCR5,CD94,CXCR3。IL18R

Th2 标志物:IL13、IL1R1、IL4、CCR8、CSF2

Th17 标记: CCR6, IL17A, IL6, RORC, IRF4

Treg 标记物:FOXP3、CD4、IL10、IL2RA、CTLA4

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Adaptive Immune Response qPCR Array (GQH-AIR) facilitates gene expression profiling of 88 key genes involved in adaptive immunity or acquired immunity. Vertebrates have two main immune systems: innate and adaptive. While the innate immune system is responsible for host defense against pathogens in a general way, the adaptive immune system is responsible for defense against particular pathogens, thereby conferring immunological memory. Brief examples of how included genes may be grouped according to their function in the adaptive immune response are shown below:

·     Antigen presentation: CANX, HLA-A, HLA-DPB1, PSMB8, TAP2

·     Th1 markers: IL12RB1, CCR5, CD94, CXCR3. IL18R

·     Th2 markers: IL13, IL1R1, IL4, CCR8, CSF2

·     Th17 markers: CCR6, IL17A, IL6, RORC, IRF4

·     Treg markers: FOXP3, CD4, IL10, IL2RA, CTLA4

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人类适应性免疫应答 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC1

CCR8

CD40LG

FOXP3

HLA-DQA1

IL12RB1

IL1R1

IRF4

NOTCH3

PTPRC

STAT6

β-actin

B

ARG1

CD19

CD80

GATA3

HLA-DQB1

IL12RB2

IL1R2

ITGAM

PDIA3

RAG1

TAP2

GAPDH

C

CANX

CD27

CD86

HAVCR2

HLA-DRA

IL13

IL2

JAK3

PSMB8

RAG2

TBX21

LDHA

D

CCL5

CD28

CSF2

HLA-A

HLA-DRB1

IL17A

IL2RA

KLRD1

PSMB9

RORA

TNF

NONO

E

CCR1

CD3D

CTLA4

HLA-B

HLA-DRB3

IL17B

IL4

LGALS1

PSME1

RORC

TNFRSF4

PPIH

F

CCR4

CD3E

CXCR3

HLA-C

IFNG

IL17C

IL5

LTA

PSME2

STAT1

TNFSF11

GDC

G

CCR5

CD3G

CXCR4

HLA-DPA1

IL10

IL18

IL6

MS4A1

PSME3

STAT3

TRAT1

PPC

H

CCR6

CD4

CXCR6

HLA-DPB1

IL12A

IL18R1

IL9

NFKB1

PTGDR2

STAT4

ZAP70

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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