ScienCellGK079人类软骨细胞生物学qPCR检测试剂盒

2023-06-29

GK079 ScienCell 人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

金山科研平台作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK079 ScienCell人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK079

产品名称:GK079 ScienCell人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK079 ScienCell人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人软骨细胞生物学 qPCR 芯片试剂盒 (GQH-CHN) 有助于对软骨细胞功能中的 88 个关键基因进行基因表达分析。 软骨细胞嵌入软骨基质中,负责产生和维持软骨基质。 软骨细胞起源于间充质,在骨化过程中终末分化时肥大。 如何根据其在软骨细胞生物学中的功能对所包含的基因进行分组的简要示例如下所示:

软骨生成: COL2A1, ACAN, SOX9, MIA, PTCH1

ECM相互作用:RHOA,ITGAV,CD44,PTK2,ITGB1

机械转导: NFATC4, TRPV4, CREB1, MAPK1, PRKCE

改造和修复:BMP2,HIF1A,MMP9,WNT9A,ICAM1

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Chondrocyte Biology qPCR Array Kit (GQH-CHN) facilitates gene expression profiling of 88 key genes in chondrocyte function. Chondrocytes are embedded in the cartilage matrix and are responsible for producing and maintaining it. Chondrocytes originate in the mesenchyme and are hypertrophic when terminally differentiated during ossification. Brief examples of how included genes may be grouped according to their function in chondrocyte biology are shown below:

·     Chondrogenesis: COL2A1, ACAN, SOX9, MIA, PTCH1

·     ECM interaction: RHOA, ITGAV, CD44, PTK2, ITGB1

·     Mechanotransduction: NFATC4, TRPV4, CREB1, MAPK1, PRKCE

·     Remodeling and repair: BMP2, HIF1A, MMP9, WNT9A, ICAM1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查询™人软骨细胞生物学 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ACAN

CACNA1C

COL2A1

FGFR3

IGF1R

ITGA6

MAPK3

NFATC4

PTGER4

RUNX2

SOX9

β-actin

B

ALPL

CACNA1D

COL4A1

FLNA

IHH

ITGA7

MATN1

NKX3-2

PTH1R

SCNN1A

SP7

GAPDH

C

ANXA5

CD151

COL4A2

FLNB

ILK

ITGAV

MATN3

OTOR

PTHLH

SCNN1B

SPARC

LDHA

D

ANXA6

CD44

CREB1

FOXC1

ITGA1

ITGB1

MATN4

PRG4

PTK2

SCNN1G

TGFB3

NONO

E

AQP1

CD47

CRTAC1

FOXC2

ITGA2

ITGB5

MIA

PRKCA

PXN

SMAD2

TNC

PPIH

F

BMP2

CHAD

CTSB

HIF1A

ITGA3

KCNA4

MMP13

PRKCB

RAC1

SMAD3

TRPV4

GDC

G

BMP4

CILP

DDR2

IBSP

ITGA4

KCNA6

MMP9

PRKCE

RARG

SOX5

VEGFA

PPC

H

CACNA1A

COL11A2

EPYC

ICAM1

ITGA5

MAPK1

NCAM1

PTCH1

RHOA

SOX6

WNT9A

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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