ScienCellGK089人类Ephrin信号qPCR检测试剂盒

2023-06-29

GK089 ScienCell 人类Ephrin信号qPCR检测试剂盒,Human Ephrin Signaling qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK089 ScienCell人类Ephrin信号qPCR检测试剂盒,Human Ephrin Signaling qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK089

产品名称:GK089 ScienCell人类Ephrin信号qPCR检测试剂盒,Human Ephrin Signaling qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK089 ScienCell人类Ephrin信号qPCR检测试剂盒,Human Ephrin Signaling qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人类ephrin信号qPCR芯片试剂盒(GQH-EPH)分析了88个参与ephrin信号传导和信号转导的关键基因。 Ephrin 信号传导参与轴突引导、血管生成、细胞迁移、胚胎发育和分割等发育过程。 肾上腺素配体和受体根据其结构和结合亲和力细分为两类:mahuangsu-A和mahuangsu-B。 ephrin 信号的一个更dute的方面是它可以双向表达为“正向信号"和“反向信号"。 如何根据其功能对所包含的基因进行分组的简要示例如下所示:

信号受体:EPHA2、EPHA4、EPHB1、EPHB2、EPHB4

配体:EFNA1、EFNA2、EFNA5、EFNB1、EFNB2

细胞骨架动力学:ACTB、ACTG1、ACTN1、CFL1、DNM1

囊泡贩运:AP2B1、AP2S1、APH1A、CAV1、CLTA

止血: FN1, FYN, ITGAVM ITGB1, LYN

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Ephrin Signaling qPCR Array Kit (GQH-EPH) profiles 88 key genes involved in ephrin signaling and signal transduction. Ephrin signaling is involved in such developmental processes as axonal guidance, angiogenesis, cell migration, embryonic development, and segmentation. Ephrin ligands and receptors are subdivided into two classes based on their structure and binding affinities: ephrin-A and ephrin-B. A more unique aspect of ephrin signaling is that it can occur bidirectionally expressing as both a “forward signal" and a “reverse signal." Brief examples of how included genes may be grouped according to their function are shown below:

· Signaling receptors: EPHA2, EPHA4, EPHB1, EPHB2, EPHB4

· Ligands: EFNA1, EFNA2, EFNA5, EFNB1, EFNB2

· Cytoskeletal dynamics: ACTB, ACTG1, ACTN1, CFL1, DNM1

· Vesicle Trafficking: AP2B1, AP2S1, APH1A, CAV1, CLTA

· Hemostasis: FN1, FYN, ITGAVM ITGB1, LYN

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人 Ephrin 信号 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTB

AP2S1

DNM1

EFNB3

EPHB2

GRIA1

LIMK1

MYL6

PIK3CG

RAP1A

TF

β-actin

B

ACTG1

APH1A

EFNA1

EPHA1

EPHB3

GRIN1

LYN

MYL9

PIK3R1

RASA1

TIAM1

GAPDH

C

ACTN1

CAV1

EFNA2

EPHA2

EPHB4

GRIN2B

MAP2K1

NCK1

PSEN2

RGS3

TLN1

LDHA

D

ADAM10

CDC42

EFNA3

EPHA3

FN1

HRAS

MAPK1

NCK2

PSENEN

RHOA

VAV2

NONO

E

AP2A1

CFL1

EFNA4

EPHA4

FYN

ITGA5

MMP2

NCSTN

PTK2

ROCK1

VAV3

PPIH

F

AP2A2

CLTA

EFNA5

EPHA5

GNAI1

ITGAV

MMP9

PAK1

PXN

SDC2

VCL

GDC

G

AP2B1

CLTB

EFNB1

EPHA7

GRB2

ITGB1

MYH10

PAK2

RAC1

SRC

VEGFA

PPC

H

AP2M1

CLTC

EFNB2

EPHB1

GRB7

KDR

MYH9

PIK3CA

RAF1

SYNJ1

WASL

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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