ScienCellGK090人体细胞连接qPCR检测试剂盒

2023-06-29

GK090 ScienCell 人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK090 ScienCell人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK090

产品名称:GK090 ScienCell人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK090 ScienCell人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人类细胞连接 qPCR 阵列试剂盒 (GQH-JXN) 分析了参与细胞连接形成、组织和维护的 88 个关键基因。 该试剂盒可研究对细胞通讯、细胞旁转运和锚定很重要的细胞-细胞连接。 如何对所包含的基因进行分类的简要示例如下所示:

间隙连接点: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1

粘附连接: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2

紧密结: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)

桥粒: DSC1, DSG1, DSP, ITGB4, JUP

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance. This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring. Brief examples of how included genes may be categorized are shown below:

· Gap junctions: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1

· Adherens junctions: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2

· Tight junctions: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)

· Desmosomes: DSC1, DSG1, DSP, ITGB4, JUP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查询™人类细胞连接 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

CADM1

CDH19

CELSR3

CLDN8

CXADR

DSP

GJA5

JAM3

NECTIN3

PECAM1

β-actin

B

ACTA2

CADM3

CDH2

CGN

CLTA

DCHS1

F11R

GJB1

JUP

OCLN

PVR

GAPDH

C

ACTB

CDH1

CDH3

CLDN1

CLTB

DCHS2

FAT1

GJB6

LEF1

PAK1

RHOA

LDHA

D

ACTC1

CDH10

CDH5

CLDN10

CLTC

DNM1

FAT2

ICAM1

MAGI1

PANX1

SNAI1

NONO

E

ACTG1

CDH11

CDX1

CLDN2

CLTCL1

DSC1

FAT3

ILK

MAGI2

PARD3

SP1

PPIH

F

ACTG2

CDH13

CDX2

CLDN4

CSNK1D

DSC2

FAT4

ITGB1

MYLK

PARD6A

SRC

GDC

G

ANG

CDH15

CELSR1

CLDN5

CTNNB1

DSG1

GJA1

ITGB4

NECTIN1

PCDH7

TJP1

PPC

H

AP2M1

CDH16

CELSR2

CLDN7

CTNND1

DSG3

GJA4

JAM2

NECTIN2

PCDH8

WNK4

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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