GK091 ScienCell 人肝星状细胞生物学qPCR检测试剂盒,Human Hepatic Stellate Cell Biology qPCR Array Kit
ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。
北京诺博莱德作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
GK091 ScienCell人肝星状细胞生物学qPCR检测试剂盒,Human Hepatic Stellate Cell Biology qPCR Array Kit,产品简介:
产品品牌: ScienCell
产品货号:GK091
产品名称:GK091 ScienCell人肝星状细胞生物学qPCR检测试剂盒,Human Hepatic Stellate Cell Biology qPCR Array Kit
产品规格:One 96-well plate, one 20ul reaction per well
GK091 ScienCell人肝星状细胞生物学qPCR检测试剂盒,Human Hepatic Stellate Cell Biology qPCR Array Kit, 产品说明:
ScienCell 的 GeneQuery™ 人肝星状细胞生物学 qPCR 芯片试剂盒 (GQH-HSB) 旨在促进 88 个关键基因的基因表达谱分析,这些基因涉及 (i) 人肝星状细胞活化和增殖;(二)视黄醇代谢;(iii)细胞外基质(ECM)合成和重塑;以及(iv)肝星状细胞相关疾病。如何根据其功能对所包含的基因进行分组的简要示例如下所示:
肝星状细胞标志物:ACTA2,GFAP,DES,NGFR,COL2A1,VIM,LAMA1,NES
肝星状细胞活化和增殖:ICAM1,MMP13,RELN,TIMP1,SYP,CDH1,RARA,PDGF,TGFB1,MMP2
肝脏发育和再生:STX2,PTN,SYNM,FOXF1,ICAM1,VCAM1,ITGB3,HGF,CXCL12,DES
视黄醇代谢: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
ECM合成和重塑:TGFB1,COL4A2,层粘连蛋白亚基,SPARC,VCAN,CTGF
细胞因子生产:EGF,IGF,PDGF,FGF,CSF1,CCL2,TNF,CXCL2,CXCL3,CXCL8
肝纤维化和癌症发展:PKHD1,TMEM67,RPGRIP1L,SLC17A5,PKD1,CC2D2A,B9D1,B9D2
GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。
ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:
· Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES
· Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2
· Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES
· Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
· ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
· Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8
· Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
基因查询™人肝星状细胞生物学qPCR阵列板布局*(8 个粗体和斜体控件)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ACTA2 | COL2A1 | CXCL2 | FGF1 | IGF1 | LAMB1 | MYH10 | NGFR | RARA | SPARC | TIMP1 | β-actin |
B | B9D1 | COL4A2 | CXCL3 | FGF2 | IGF2 | LAMB3 | MYH11 | PDGFA | RBP1 | SPP1 | TMEM67 | GAPDH |
C | B9D2 | CRABP1 | CXCL8 | FOXF1 | IL1A | LAMC1 | MYH9 | PDGFB | RBP2 | STX2 | TNC | LDHA |
D | BDNF | CRABP2 | CYP26A1 | GFAP | ITGB3 | LEP | NCAM1 | PDGFC | RBP4 | SYNM | TNF | NONO |
E | CC2D2A | CSF1 | CYP26B1 | HAS1 | KLF6 | LHX2 | NCAM2 | PDGFD | RBP5 | SYP | VCAM1 | PPIH |
F | CCL2 | CTGF | DES | HGF | LAMA1 | LRAT | NES | PKD1 | RELN | TGFB1 | VCAN | GDC |
G | CDH1 | CXCL10 | EGF | ICAM1 | LAMA2 | MMP13 | NFKB1 | PKHD1 | RPGRIP1L | TGFBI | VIM | PPC |
H | CLEC3B | CXCL12 | F2 | IFNG | LAMA3 | MMP2 | NFKB2 | PTN | SLC17A5 | THBS2 | VTN | NTC |
提示:基因选择可能会根据新的研究和开发进行更新
ScienCell基因表达分析产品订购信息:
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