ScienCellGK093 Human Renal Mesangial Cell Biology

2023-06-29

GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

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GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK093

产品名称:GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人肾系膜细胞生物学qPCR阵列试剂盒(GQH-RMB)旨在促进参与基本系膜细胞生物学和肾小球生理学和病理生理学功能的88个关键基因的基因表达谱分析。如何根据其功能对所包含的基因进行分组的简要示例如下所示:

肾系膜细胞标志物:ACTA1,FN1,PDGFRB,TNS1,ITGA8

系膜基质组装:SERPINB7、胶原蛋白 4/5、层粘连蛋白 α/β/γ、SDC2、HSPG2、CSPG5、NID1、ITGA8

生长因子调节: IGF1, PTN, MFGE8, GDF10, CTGF, NGF, PSEN2, SH3KBP1, ADAM12, AFAP1L2, SHC3

血管生成: VCAM1, ANGPT2, HOPX, MYH11, NRP2, HDAC9, GJA5, ADAMTS1, FHL2, PLN, CTGF

内吞作用:CLTA, CLTB, CLTC, AP2A1, AP2A2, AP2B1, AP2M1, AP2S1, SMAD2, SMAD3, ZFYVE9, TGFB 受体

跨膜转运蛋白活性: KCNMB1, GJA5, CACNA2D1, ABCC9, P2RX1, KCNRG, TRPCs, KCNQ5, PIEZO2

与以下因素有关的基因

系膜增生性肾小球肾炎:SERPINB7、IL6、CD79A、CCL2、FGF2、EDN1、CCR5、GAS6、AXL

弥漫性系膜硬化症:ACTN4、TRPC6、INF2、WT1、PLCE1、PAX2、ARHGDIA、LAMB2、LAMC1

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Renal Mesangial Cell Biology qPCR Array Kit (GQH-RMB) is designed to facilitate gene expression profiling of 88 key genes involved in essential mesangial cell biology and functions involved in glomerular physiology and pathophysiology. Brief examples of how included genes may be grouped according to their functions are shown below:

· Renal mesangial cell markers: ACTA1, FN1, PDGFRB, TNS1, ITGA8

· Mesangial matrix assembly: SERPINB7, Collagen type 4/5, Laminin α/β/γ, SDC2, HSPG2, CSPG5, NID1, ITGA8

· Growth factor modulation: IGF1, PTN, MFGE8, GDF10, CTGF, NGF, PSEN2, SH3KBP1, ADAM12, AFAP1L2, SHC3

· Vasculogenesis: VCAM1, ANGPT2, HOPX, MYH11, NRP2, HDAC9, GJA5, ADAMTS1, FHL2, PLN, CTGF

· Endocytosis: CLTA, CLTB, CLTC, AP2A1, AP2A2, AP2B1, AP2M1, AP2S1, SMAD2, SMAD3, ZFYVE9, TGFB receptors

· Transmembrane transporter activity: KCNMB1, GJA5, CACNA2D1, ABCC9, P2RX1, KCNRG, TRPCs, KCNQ5, PIEZO2

· Genes implicated in

o Mesangial proliferative glomerulonephritis: SERPINB7, IL6, CD79A, CCL2, FGF2, EDN1, CCR5, GAS6, AXL

o Diffuse mesangial sclerosis: ACTN4, TRPC6, INF2, WT1, PLCE1, PAX2, ARHGDIA, LAMB2, LAMC1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人肾系膜细胞生物学qPCR阵列板布局*(8 个粗体和斜体控件)

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC9

AP2A1

CCL2

COL5A1

FHL2

IGF1

LAMA2

MFGE8

PDGFRB

SFRP2

TGFBR3

β-actin

B

ACTA1

AP2A2

CCR5

COL5A2

FN1

IL6

LAMA3

MYH11

PIEZO2

SH3KBP1

TNS1

GAPDH

C

ACTN4

AP2B1

CD79A

COL5A3

GAS6

INF2

LAMA4

NGF

PLCE1

SHC3

TRPC1

LDHA

D

ADAM12

AP2M1

CLTA

CSPG5

GDF10

ITGA8

LAMA5

NID1

PLN

SMAD1

TRPC4

NONO

E

ADAMTS1

AP2S1

CLTB

CTGF

GJA5

KCNMB1

LAMB1

NID2

PSEN2

SMAD2

TRPC6

PPIH

F

AFAP1L2

ARHGDIA

CLTC

EDN1

HDAC9

KCNQ5

LAMB2

NRP2

PTN

SMAD3

VCAM1

GDC

G

AGTR1

AXL

COL4A1

ETS1

HOPX

KCNRG

LAMC1

P2RX1

SDC2

TGFBR1

WT1

PPC

H

ANGPT2

CACNA2D1

COL4A2

FGF2

HSPG2

LAMA1

MECOM

PAX2

SERPINB7

TGFBR2

ZFYVE9

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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