ScienCellGK094Human Mesenchymal Stem Cell Differentiation qPCR ArrayKit

2023-06-29

GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK094

产品名称:GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人间充质干细胞分化 qPCR 阵列试剂盒 (GQH-MSC) 旨在促进与间充质干细胞 (MSC) 生物学、多能性维持和分化相关的 88 个关键基因的基因表达谱分析。下面显示了如何对所包含的基因进行分组的简要示例:

MSC 标记:GNL3、BMPR1A、BMPR1B、BMPR2、KIT、NT5E、ENG、ALCAM、ANPEP、CDH2、CD44、PTPRC、THY1、HLA-A

干性标记:FUT4、PROM1、ABCG2、SOX2、FGF2、INS、LIF

脂肪生成标志物:TNFRSF9,ADIPOQ,ADIPOR1 / 2,CIDEA,CLTCL1,DLK2,FABP4,SLC27As,SLC2A4,LEP,PLIN2

软骨生成标志物:ACAN,ANXA6,CD151,CHAD,FOXC1/2,MATN1/3/4,MIA,OTOR,SOX5/6/9

肌源性前体标志物:CDH15,CAV1,CD34,FOXK1,MYF5,MYOD1,MYOG,NCAM1,PAX3/7/9

骨祖标志物:ALPP,DCN,MCAM,MEPE,SP7,RUNX2,THPO

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Differentiation qPCR Array Kit (GQH-MSC) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) biology, pluripotency maintenance, and differentiation. Brief examples of how included genes may be grouped are shown below:

· MSC markers: GNL3, BMPR1A, BMPR1B, BMPR2, KIT, NT5E, ENG, ALCAM, ANPEP, CDH2, CD44, PTPRC, THY1, HLA-A

· Stemness markers: FUT4, PROM1, ABCG2, SOX2, FGF2, INS, LIF

· Adipogenesis markers: TNFRSF9, ADIPOQ, ADIPOR1/2, CIDEA, CLTCL1, DLK2, FABP4, SLC27As, SLC2A4, LEP, PLIN2

· Chondrogenesis markers: ACAN, ANXA6, CD151, CHAD, FOXC1/2, MATN1/3/4, MIA, OTOR, SOX5/6/9

· Myogenic precursor markers: CDH15, CAV1, CD34, FOXK1, MYF5, MYOD1, MYOG, NCAM1, PAX3/7/9

· Osteoprogenitor markers: ALPP, DCN, MCAM, MEPE, SP7, RUNX2, THPO

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人间充质干细胞分化qPCR阵列板布局*(8 个粗体和斜体控件)

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCG2

ANXA6

CD44

DCN

FOXC2

ITGA1

MATN4

NGFR

PPARG

SLC27A6

TFRC

β-actin

B

ACAN

BMPR1A

CDH15

DLK1

FOXK1

ITGB1

MCAM

NT5E

PPARGC1A

SLC2A4

THPO

GAPDH

C

ADIPOQ

BMPR1B

CDH2

DLK2

FUT4

ITM2A

MEPE

OTOR

PROM1

SOX2

THY1

LDHA

D

ADIPOR1

BMPR2

CHAD

ENG

GNL3

KIT

MIA

PAX3

PTPRC

SOX5

TNFRSF9

NONO

E

ADIPOR2

BSCL2

CIDEA

FABP4

HLA-A

LEP

MYF5

PAX7

RUNX2

SOX6

UCP1

PPIH

F

ALCAM

CAV1

CLTCL1

FAM20B

IBSP

LIF

MYOD1

PAX9

SLC27A1

SOX9

VCAM1

GDC

G

ALPP

CD151

COL4A1

FGF2

ICAM1

MATN1

MYOG

PDGFRA

SLC27A2

SP7

VIM

PPC

H

ANPEP

CD34

CRTAC1

FOXC1

INS

MATN3

NCAM1

PLIN2

SLC27A4

SUSD2

ZIC1

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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