GK095 ScienCell 人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit
ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。
北京诺博莱德作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
GK095 ScienCell人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit,产品简介:
产品品牌: ScienCell
产品货号:GK095
产品名称:GK095 ScienCell人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit
产品规格:One 96-well plate, one 20ul reaction per well
北京裕恒丰科技有限公司是ScienCell中国区总代理,提供各种基因表达分析相关品,ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。
GK095 ScienCell人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit, 产品说明:
ScienCell 的 GeneQuery™ 人间充质到上皮转化 qPCR 阵列试剂盒 (GQH-MET) 旨在促进参与可逆间充质到上皮转化 (MET) 的 88 个关键基因的基因表达谱分析。MET和反向过程,上皮到间充质转化(EMT),参与胚胎发生,癌症转移和干细胞重编程。下面显示了如何对基因进行分组的简要示例:
EMT标记: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD
EMT-MET过渡:PAX2,WT1,BMP7,TWIST1,PRRX1
增殖和形态发生控制:AKT1,BMP1,BMP7,EGFR,FGFBP1,IGFBP4,JAG1,PDGFRB,SNAI1,SOX10,VCAN,WNT5A,WNT11,ZEB1
分化控制: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, 节点, SMAD2, SOX10, WNT5B
调节 MET 的转录因子:CTNNB1、ESR1、FOXC2、NOTCH1、SMAD2、SNAI1/2/3、STAT3、TCF3、TCF4、TWIST1、ZEB2
MET中涉及的信号通路
雌激素受体:CAV2,ESR1,KRT19,TGFB3
GPCR: AKT1, FZD7, GNG11, RAC1, RGS2
PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5
GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。
ScienCell's GeneQuery™ Human Mesenchymal To Epithelial Transition qPCR Array Kit (GQH-MET) is designed to facilitate gene expression profiling of 88 key genes involved in reversible mesenchymal to epithelial transition (MET). MET and the reverse process, epithelial to mesenchymal transition (EMT), are involved in embryogenesis, cancer metastasis, and stem cell reprogramming. Brief examples of how genes may be grouped are shown below:
· EMT markers: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD
· EMT-MET transition: PAX2, WT1, BMP7, TWIST1, PRRX1
· Proliferation and morphogenesis control: AKT1, BMP1, BMP7, EGFR, FGFBP1, IGFBP4, JAG1, PDGFRB, SNAI1, SOX10, VCAN, WNT5A, WNT11, ZEB1
· Differentiation control: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, NODAL, SMAD2, SOX10, WNT5B
· Transcription factors regulating MET: CTNNB1, ESR1, FOXC2, NOTCH1, SMAD2, SNAI1/2/3, STAT3, TCF3, TCF4, TWIST1, ZEB2
· Signaling pathways involved in MET
o Estrogen receptor: CAV2, ESR1, KRT19, TGFB3
o GPCR: AKT1, FZD7, GNG11, RAC1, RGS2
o PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
基因查询™人间充质到上皮过渡 qPCR 阵列板布局*(8 个粗体和斜体控件)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ACTA2 | CAV2 | COL3A1 | F11R | IGFBP4 | MUC1 | PIK3CG | RGS2 | SNAI3 | TGFB2 | VCAN | β-actin |
B | AKT1 | CDH1 | COL5A2 | FGFBP1 | ILK | NODAL | PIK3R1 | RPS27A | SOX10 | TGFB3 | VIM | GAPDH |
C | AKT2 | CDH11 | CTNNB1 | FN1 | JAG1 | NOTCH1 | PIK3R3 | S100A4 | SPINT2 | TGFBR1 | WNT11 | LDHA |
D | ARHGEF18 | CDH2 | DSP | FOXC2 | KRT14 | PAX2 | PIK3R5 | SDC1 | STAT3 | TGFBR2 | WNT5A | NONO |
E | AXL | CDKN2A | EGFR | FZD7 | KRT19 | PDGFD | PRKCZ | SMAD2 | STK11 | TIMP1 | WNT5B | PPIH |
F | BMP1 | CDKN2B | ERBB3 | GNG11 | LEF1 | PDGFRB | PRRX1 | SMURF1 | TCF3 | TRIM28 | WT1 | GDC |
G | BMP2 | CLDN4 | ESR1 | GSC | MMP2 | PIK3CA | RAB25 | SNAI1 | TCF4 | TWIST1 | ZEB1 | PPC |
H | BMP7 | CLDN7 | ETS1 | HNRNPAB | MST1R | PIK3CD | RAC1 | SNAI2 | TGFB1 | UBA52 | ZEB2 | NTC |
提示:基因选择可能会根据新的研究和开发进行更新
ScienCell基因表达分析产品订购信息:
GK001 人类细胞凋亡qPCR检测试剂盒,Human Apoptosis qPCR Array Kit
GK002 人类多能干细胞qPCR检测试剂盒,Human Pluripotent Stem Cell qPCR Array Kit
GK003 人类细胞周期qPCR检测试剂盒,Human Cell Cycle qPCR Array Kit
GK005 人上皮细胞生物学qPCR检测试剂盒,Human Epithelial Cell Biology qPCR Array Kit
GK006 人类神经发育和再生qPCR检测试剂盒,Human Neural Development and Regeneration qPCR Array Kit
GK007 人类神经可塑性qPCR检测试剂盒,Human Neural Plasticity qPCR Array Kit
GK008 人类神经传递和膜转运qPCR检测试剂盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit
GK009 人类星型胶质细胞生物学qPCR检测试剂盒,Human Astrocyte Cell Biology qPCR Array Kit
GK010 人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit
GK011 人类黑素细胞生物学qPCR检测试剂盒,Human Melanocyte Cell Biology qPCR Array Kit
GK013 人类星型胶质细胞转录因子qPCR检测试剂盒,Human Astrocyte Transcription Factors qPCR Array Kit
GK017 人血管生成qPCR检测试剂盒,Human Angiogenesis qPCR Array Kit
GK018 人内皮细胞表型异质性qPCR检测试剂盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit
GK019 人内皮细胞生物学qPCR检测试剂盒,Human Endothelial Cell Biology qPCR Array Kit
GK020 人B细胞受体信号通路qPCR检测试剂盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit
GK021 人类T细胞受体信号通路qPCR检测试剂盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit
GK022 人内吞作用qPCR检测试剂盒,Human Endocytosis qPCR Array Kit
GK023 人皮内细胞分化qPCR检测试剂盒,Human Endothelial Cell Differentiation qPCR Array Kit
GK024 人体剪切应力和机械传导qPCR检测试剂盒,Human Shear Stress and Mechanotransduction qPCR Array Kit
GK025 人类Notch信号通路qPCR检测试剂盒,uman Notch Signaling Pathway qPCR Array Kit
GK026 人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit
GK027 人类非经典Wnt信号通路qPCR检测试剂盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit
GK028 人类Wnt信号靶基因qPCR检测试剂盒,Human Wnt Signaling Target Genes qPCR Array Kit
GK029 Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit
GK034 人肺纤维化qPCR检测试剂盒,Human Pulmonary Fibrosis qPCR Array Kit
GK035 人体缺氧反应qPCR检测试剂盒,Human Hypoxia Response qPCR Array Kit
GK036 人类HIF1信号响应qPCR检测试剂盒,Human HIF1 Signaling Response qPCR Array Kit
GK037 人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit
GK038 人类吞噬作用qPCR检测试剂盒,Human Phagocytosis qPCR Array Kit
GK039 人类先天免疫反应qPCR检测试剂盒,Human Innate Immune Response qPCR Array Kit
GK040 人成纤维细胞生长因子信号通路qPCR检测试剂盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit
GK041 人体皮肤伤口愈合qPCR检测试剂盒,Human Skin Wound Healing qPCR Array Kit
GK056 人体止血qPCR检测试剂盒,Human Hemostasis qPCR Array Kit
GK060 人类昼夜节律qPCR检测试剂盒,Human Circadian Rhythm qPCR Array Kit
GK061 人类DNA损伤感应qPCR检测试剂盒,Human DNA Damage Sensing qPCR Array Kit
GK062 人类细胞生长和分裂qPCR检测试剂盒,Human Cell Growth and Division qPCR Array Kit
GK063 人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit
GK069 人类小胶质细胞生物学qPCR检测试剂盒,Human Microglia Cell Biology qPCR Array Kit
GK072 人类适应性免疫反应qPCR检测试剂盒,Human Adaptive Immune Response qPCR Array Kit
GK073 人类小胶质细胞Sensome qPCR检测试剂盒,Human Microglial Sensome qPCR Array Kit
GK074 人角质细胞生物学qPCR检测试剂盒,Human Keratocyte Biology qPCR Array
GK078 人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit
GK079 人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit
GK080 人成骨分化qPCR检测试剂盒,GeneQuery™ Human Osteogenic Differentiation qPCR Array Kit
GK081 人类周细胞生物学qPCR检测试剂盒,Human Pericyte Biology qPCR Array Kit
GK084 人体轴突导向qPCR检测试剂盒,Human Axon Guidance qPCR Array kit
GK087 人类染色质组织和重塑qPCR检测试剂盒,Human Chromatin Organization and Remodeling qPCR Array Kit
GK088 人VEGF信号通路qPCR检测试剂盒,Human VEGF Signaling Pathway qPCR Array Kit
GK089 人类Ephrin信号qPCR检测试剂盒,Human Ephrin Signaling qPCR Array Kit
GK090 人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit
GK091 人肝星状细胞生物学qPCR检测试剂盒,Human Hepatic Stellate Cell Biology qPCR Array Kit
GK092 人胰腺星状细胞生物学qPCR检测试剂盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit
GK093 Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit
GK094 Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit
GK095 人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit
GK096 人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit
GK097 人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit
GK098 人类心脏肌细胞生物学qPCR检测试剂盒,Human Cardiac Myocyte Biology qPCR Array Kit
GK099 人类骨骼肌细胞生物学qPCR检测试剂盒,Human Skeletal Muscle Cell Biology qPCR Array Kit
GK100 人角质形成细胞生物学qPCR检测试剂盒,Human Keratinocyte Cell Biology qPCR Array Kit
GK101 人类Synoviocyte细胞生物学qPCR检测试剂盒,Human Synoviocyte Cell Biology qPCR Array Kit
GK102 人类滋养层细胞生物学qPCR检测试剂盒,Human Trophoblast Cell Biology qPCR Array Kit
GK103 人前脂肪细胞生物学qPCR检测试剂盒,Human Preadipocyte Cell Biology qPCR Array Kit
GK104 人类细胞骨架重塑qPCR检测试剂盒,Human Cytoskeletal Remodeling qPCR Array Kit
GK105 人类ECM降解qPCR检测试剂盒,Human ECM Degradation qPCR Array Kit
GK106 人类焦点粘附复合物qPCR检测试剂盒,Human Focal Adhesion Complexes qPCR Array Kit
GK107 人内皮素信号qPCR检测试剂盒,Human Endothelin Signaling qPCR Array Kit
GK111 人凝集素调节免疫应答qPCR检测试剂盒,Human Lectin Regulation in Immune Response qPCR Array Kit
GK112 人类神经分化标记qPCR检测试剂盒,Human Neural Differentiation Markers qPCR Array Kit
GK113 人类神经干细胞标记qPCR检测试剂盒,Human Neural Stem Cell Markers qPCR Array Kit
GK114 人类小胶质细胞极化标记qPCR检测试剂盒,Human Microglial Polarization Markers qPCR Array Kit
GK115 人类骨髓谱系标记qPCR检测试剂盒,Human Myeloid Lineage Markers qPCR Array Kit
GK116 人B细胞和T细胞发育标记qPCR检测试剂盒,Human B and T Cells Development Markers qPCR Array Kit
GK117 人胸腺细胞发育标记qPCR检测试剂盒,Human Thymocyte Development Markers qPCR Array Kit
GK118 人类先天淋巴细胞发育标记qPCR检测试剂盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit
GK119 人类神经祖细胞标记qPCR检测试剂盒,Human Neural Progenitor Markers qPCR Array Kit
GK121 人PD-1/PD-L1检查点途径qPCR检测试剂盒,GeneQuery™ Human PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit
GK123 人类生长因子qPCR检测试剂盒,Human Growth Factors qPCR Array Kit
GK124 人类转录因子qPCR检测试剂盒,Human Transcription Factors qPCR Array Kit
GK125 人干细胞转录因子qPCR检测试剂盒,Human Stem Cell Transcription Factors qPCR Array Kit
GK126 人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit
GK127 人脑发育标记物qPCR检测试剂盒,Human Brain Development Markers qPCR Array Kit
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