ScienCellGK097人类平滑肌细胞生物学qPCR检测的特点

2023-06-29

GK097 ScienCell 人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK097 ScienCell人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK097

产品名称:GK097 ScienCell人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

北京裕恒丰科技有限公司是ScienCell中国区总代理,提供各种基因表达分析相关品,ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK097 ScienCell人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人平滑肌细胞生物学 qPCR 阵列试剂盒 (GQH-SMC) 旨在促进参与人类平滑肌细胞 (SMC) 生物学的 88 个关键基因的基因表达谱分析,例如血管 SMC 收缩、SMC 表型切换和细胞外基质 (ECM) 合成。与选定的SMC相关疾病有关的基因也包含在阵列中。下面显示了如何根据其功能对基因进行分组的简要示例:

SMC 标记: ACTA2, CDH5, CALD1, HEXIM1, HRH2, MLNR, TAGLN, DES, CSPG4, SMTN, MKL1/2

血管 SMC 收缩: ANO1, ADRA1, NPR1/2, GNAs, MYLKs, ADORA2s, EDNRA, MYH11, MYLs

SMC 表型切换: MYH11, MEF2B, SRF, ELK1, MYOCD, GATA6, MKL1/2, RHOA, TGFB1, NOTCH1/3, HEY2

SMC 异质性: ACTA2, TAGLN, CNN2, MYH11, OGN, AQP1, PLN, MGP, SPP1, KDR, SMTN, VCL, DES

ECM合成:LAMA5,胶原蛋白,ELN,OGN,MGP,SPP1,FGA/B / G

与以下因素有关的基因

全身性高血压:PTGIS、AGT、ADD1、AGTR1、GNB3、NOS3

贲门失弛缓症: NOS1, AAAS, GMPPA, GUCY1A3, HLA-DQA1/B1

支气管痉挛/哮喘:HNMT、IL13、CCL11、ADRB2、ALOX5、SCGB3A2

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Smooth Muscle Cell Biology qPCR Array Kit (GQH-SMC) is designed to facilitate gene expression profiling of 88 key genes involved in human smooth muscle cell (SMC) biology, such as vascular SMC contraction, SMC phenotypic switching, and the extracellular matrix (ECM) synthesis. Genes implicated in selected SMC related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:

· SMC markers: ACTA2, CDH5, CALD1, HEXIM1, HRH2, MLNR, TAGLN, DES, CSPG4, SMTN, MKL1/2

· Vascular SMC contraction: ANO1, ADRA1s, NPR1/2, GNAs, MYLKs, ADORA2s, EDNRA, MYH11, MYLs

· SMC phenotypic switching: MYH11, MEF2B, SRF, ELK1, MYOCD, GATA6, MKL1/2, RHOA, TGFB1, NOTCH1/3, HEY2

· SMC heterogeneity: ACTA2, TAGLN, CNN2, MYH11, OGN, AQP1, PLN, MGP, SPP1, KDR, SMTN, VCL, DES

· ECM synthesis: LAMA5, collagens, ELN, OGN, MGP, SPP1, FGA/B/G

· Genes implicated in

o systemic hypertension: PTGIS, AGT, ADD1, AGTR1, GNB3, NOS3

o achalasia: NOS1, AAAS, GMPPA, GUCY1A3, HLA-DQA1/B1

o bronchospasm/asthma: HNMT, IL13, CCL11, ADRB2, ALOX5, SCGB3A2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人平滑肌细胞生物学qPCR阵列板布局*(8 个粗体和斜体控件)

1

2

3

4

5

6

7

8

9

10

11

12

A

AAAS

ADRB2

AVPR1B

COL3A1

ELN

GNA13

HRH2

MLNR

MYOCD

NPR2

SCGB3A2

β-actin

B

ACTA2

AEBP1

CALCRL

COL4A1

FGA

GNB3

IL13

MYH11

NKX3-1

OGN

SMAD3

GAPDH

C

ADD1

AGT

CALD1

CSPG4

FGB

GUCY1A3

KDR

MYL6

NOS1

PLN

SMTN

LDHA

D

ADORA2A

AGTR1

CCL11

CYP4A11

FGG

HEXIM1

LAMA5

MYL6B

NOS3

PTGIR

SPP1

NONO

E

ADORA2B

ALOX5

CDH5

CYP4A22

GATA6

HEY2

MEF2B

MYL9

NOTCH1

PTGIS

SRF

PPIH

F

ADRA1A

ANO1

CNN2

DES

GMPPA

HLA-DQA1

MGP

MYLK

NOTCH3

RBP1

TAGLN

GDC

G

ADRA1B

AQP1

COL1A1

EDNRA

GNA11

HLA-DQB1

MKL1

MYLK2

NOX4

RBPJ

TGFB1

PPC

H

ADRA1D

AVPR1A

COL1A2

ELK1

GNA12

HNMT

MKL2

MYLK3

NPR1

RHOA

VCL

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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