ScienCellGK112人类神经分化标记qPCR检测试剂盒

2023-06-29

GK112 ScienCell 人类神经分化标记qPCR检测试剂盒,Human Neural Differentiation Markers qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK112 ScienCell人类神经分化标记qPCR检测试剂盒,Human Neural Differentiation Markers qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK112

产品名称:GK112 ScienCell人类神经分化标记qPCR检测试剂盒,Human Neural Differentiation Markers qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK112 ScienCell人类神经分化标记qPCR检测试剂盒,Human Neural Differentiation Markers qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人类神经分化标记 qPCR 阵列试剂盒 (GQH-NDM) 旨在促进参与神经分化为特定神经谱系的 40 个标记基因的基因表达谱分析。如何根据其功能对所包含的基因进行分组的简要示例如下所示:

亚成熟神经元标志物:DCX、神经D1、STMN1、TUBB3

成熟神经元标记:MAP2,NEFH,NEFM,RBFOX3

星形胶质细胞标志物:GFAP,GLUL,S100B,SLC1A2,S100A6,NFIX,NFIC

雪旺细胞标志物:GAP43、MPZ、NCAM1、S100A1、NGFR、EGR2、POU3F1

少突胶质细胞标志物:CSPG4,MBP,MOG,OLIG1,OLIG2,PDGFRA,SOX10

径向神经胶质细胞标志物:CDH2、FABP7、GFAP、HES1、HES5、NES、PAX6、VIM

神经上皮细胞标志物:CDH1、HES1、NES、NOTCH1、OCLN、SOX2

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Neural Differentiation Markers qPCR Array Kit (GQH-NDM) is designed to facilitate gene expression profiling of 40 marker genes involved in neural differentiation to specific neural lineages. Brief examples of how included genes may be grouped according to their functions are shown below:

· Immature neuron markers:DCX, NEUROD1, STMN1, TUBB3

· Mature neuron markers:MAP2, NEFH, NEFM, RBFOX3

· Astrocyte markers:GFAP, GLUL, S100B, SLC1A2, S100A6, NFIX, NFIC

· Schwann cell markers:GAP43, MPZ, NCAM1, S100A1, NGFR, EGR2, POU3F1

· Oligodendrocyte markers:CSPG4, MBP, MOG, OLIG1, OLIG2, PDGFRA, SOX10

· Radial glial cell markers:CDH2, FABP7, GFAP, HES1, HES5, NES, PAX6, VIM

· Neuroepithelial cell markers:CDH1, HES1, NES, NOTCH1, OCLN, SOX2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人类神经分化标记 qPCR 阵列板布局*(8 个粗体和斜体控件)

1

2

3

4

5

6

7

8

9

10

11

12

A

CDH1

GLUL

NEFH

OCLN

S100A6

β-actin

CDH1

GLUL

NEFH

OCLN

S100A6

β-actin

B

CDH2

HES1

NEFM

OLIG1

S100B

GAPDH

CDH2

HES1

NEFM

OLIG1

S100B

GAPDH

C

CSPG4

HES5

NES

OLIG2

SLC1A2

LDHA

CSPG4

HES5

NES

OLIG2

SLC1A2

LDHA

D

DCX

MAP2

NEUROD1

PAX6

SOX10

NONO

DCX

MAP2

NEUROD1

PAX6

SOX10

NONO

E

EGR2

MBP

NFIC

PDGFRA

SOX2

PPIH

EGR2

MBP

NFIC

PDGFRA

SOX2

PPIH

F

FABP7

MOG

NFIX

POU3F1

STMN1

GDC

FABP7

MOG

NFIX

POU3F1

STMN1

GDC

G

GAP43

MPZ

NGFR

RBFOX3

TUBB3

PPC

GAP43

MPZ

NGFR

RBFOX3

TUBB3

PPC

H

GFAP

NCAM1

NOTCH1

S100A1

VIM

NTC

GFAP

NCAM1

NOTCH1

S100A1

VIM

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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