GK117 ScienCell 人胸腺细胞发育标记qPCR检测试剂盒，Human Thymocyte Development Markers qPCR Array Kit

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理，咨询微信：jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约代理商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK117 ScienCell人胸腺细胞发育标记qPCR检测试剂盒，Human Thymocyte Development Markers qPCR Array Kit，产品简介：

产品品牌： ScienCell

产品货号：GK117

产品名称：GK117 ScienCell人胸腺细胞发育标记qPCR检测试剂盒，Human Thymocyte Development Markers qPCR Array Kit

产品规格：One 96-well plate, one 20ul reaction per well

GK117 ScienCell人胸腺细胞发育标记qPCR检测试剂盒，Human Thymocyte Development Markers qPCR Array Kit， 产品说明：

ScienCell 的 GeneQuery™ 人类胸腺细胞发育标记 qPCR 阵列试剂盒 （GQH-TDM） 旨在促进胸腺生成中涉及的 40 个标记基因的基因表达谱分析。 严格调控的常见淋巴祖细胞（CLP）谱系由几个基于细胞表面标志物表达的成熟期组成，包括双阴性阶段（CD4-和CD8-），双阳性阶段（CD4+和CD8+）和单阳性阶段（CD4+或CD8+）。CD4 + T细胞可以进一步分化为许多效应子亚群，如Th1，Th2，Th9，Th17和Tfh。

注意：所有基因名称都遵循人类基因组组织基因命名委员会（HGNC）的guanfang符号。

CLP 标记： MME， CD34， PTPRC， IL7R， FLT3

双阴性胸腺细胞标志物：CD1A、CD3D、CD3E、CD3G、CD5、CD7、CD200、IL2RA

双阳性胸腺细胞标志物：CD1A、CD4、CD5、CD7、CD8A、CD8B、CD38、SELL、CD200

CD4+ T 细胞标志物： CD3D， CD3E， CD3G， CD4， PTPRC， IL2RA， 出售

CD8+ T 细胞标志物：CD3D、CD3E、CD3G、CD8A、CD8B、GZMB

调节性T细胞标志物：IL2RA，ENTPD1，NT5E，ITGAE，FOXP3

T细胞效应亚群的标志物

Th1： FASLG， CXCR3， CCR5， IL12RB1， IL18R1

Th2： CXCR3， CCR3， CCR4， CCR8， PTGDR2

Th9： CCR6， IL9

Th17： PTPRC， IL6R， KLRB1， CCR4， CCR6， IL13RA1， IL21R

Tfh： CXCR5， PDCD1

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有说明，否则引物组可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Thymocyte Development Markers qPCR Array Kit (GQH-TDM) is designed to facilitate gene expression profiling of 40 marker genes involved in thymopoiesis. The tightly regulated lineages of common lymphoid progenitors (CLPs) consist of several maturational stages based on the expression of cell surface markers, including double negative stage (CD4- and CD8-), double positive stage (CD4+ and CD8+), and single positive stage (CD4+ or CD8+). The CD4+ T cells can further differentiate into a number of effectors subsets such as Th1, Th2, Th9, Th17, and Tfh. Brief examples of how included genes may be grouped according to their functions are shown below:

Note:all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

· CLP markers:MME, CD34, PTPRC, IL7R, FLT3

· Double negative thymocyte markers:CD1A, CD3D, CD3E, CD3G, CD5, CD7, CD200, IL2RA

· Double positive thymocyte markers:CD1A, CD4, CD5, CD7, CD8A, CD8B, CD38, SELL, CD200

· CD4+ T cell markers:CD3D, CD3E, CD3G, CD4, PTPRC, IL2RA, SELL

· CD8+ T cell markers:CD3D, CD3E, CD3G, CD8A, CD8B, GZMB

· Regulatory T cell markers:IL2RA, ENTPD1, NT5E, ITGAE, FOXP3

· Markers of T cell effector subsets

o Th1:FASLG, CXCR3, CCR5, IL12RB1, IL18R1

o Th2:CXCR3, CCR3, CCR4, CCR8, PTGDR2

o Th9:CCR6, IL9

o Th17:PTPRC, IL6R, KLRB1, CCR4, CCR6, IL13RA1, IL21R

o Tfh:CXCR5, PDCD1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人胸腺细胞发育标志物qPCR阵列板布局*8 个粗体和斜体控件）

注意：所有基因名称都遵循HGNC的guanfang符号

提示：基因选择可能会根据新的研究和开发进行更新

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