ScienCellGK125人干细胞转录因子qPCR检测试剂盒

2023-06-29

GK125 ScienCell 人干细胞转录因子qPCR检测试剂盒,Human Stem Cell Transcription Factors qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞科研金山平台现货促销授权一级代理,咨询微信:jinshanbio 金山禾斗石开平台总代理的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK125 ScienCell人干细胞转录因子qPCR检测试剂盒,Human Stem Cell Transcription Factors qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK125

产品名称:GK125 ScienCell人干细胞转录因子qPCR检测试剂盒,Human Stem Cell Transcription Factors qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK125 ScienCell人干细胞转录因子qPCR检测试剂盒,Human Stem Cell Transcription Factors qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人类干细胞转录因子qPCR阵列试剂盒(GQH-SCT)调查了与干细胞维持和分化相关的88种转录因子。转录因子是通过结合基因组的特定区域(称为增强子或消音器)来调节靶基因转录水平的蛋白质。下面显示了如何根据其功能对基因进行分组的简要示例:

多能性转录因子:NANOG、POU5F1、SOX2

胚胎发育:EOMES,FOXC2 / D3 / O1,HOXA9 / A10,KLF2 / 5,LIN28B,MAX,PITX2,SMAD1,TBX5,ZIC1

胚层形成和分化:FOXA2、GATA6、HAND1、ISL1、KLF4、SMAD2、SOX9

器官形态发生: EZH2, HOXA11/B3/B5/C9, MSX2, MYC, PAX5/8, VDR

血管生成: CDX2, JUN, NR2F2, RUNX1, WT1

造血和成骨: EGR3, ESR1, GATA1/2/3, GLI2, NFATC1, PAX9, RB1, SOX6, SP1, STAT1

神经干细胞维持和分化:ATF2、CREB1、FOSB、FOXO3、HES1、HOXD10、MCM2/7、MEF2C、NEUROD1/G1、OLIG1/2、PAX3/6、POU4F1、PPARG、SMAD3/4、SNAI1、STAT3

注意:所有基因名称都遵循人类基因组组织基因命名委员会(HGNC)的guanfang符号。

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Stem Cell Transcription Factors qPCR Array Kit (GQH-SCT) surveys a panel of 88 transcription factors related to stem cell maintenance and differentiation. Transcription factors are proteins that can regulate target gene transcription level by binding to specific regions of the genome known as enhancers or silencers. Brief examples of how genes may be grouped according to their functions are shown below:

· Pluripotency transcription factors:NANOG, POU5F1, SOX2

· Embryonic development:EOMES, FOXC2/D3/O1, HOXA9/A10, KLF2/5, LIN28B, MAX, PITX2, SMAD1, TBX5, ZIC1

· Germ layer formation and differentiation:FOXA2, GATA6, HAND1, ISL1, KLF4, SMAD2, SOX9

· Organ morphogenesis:EZH2, HOXA11/B3/B5/C9, MSX2, MYC, PAX5/8, VDR

· Angiogenesis:CDX2, JUN, NR2F2, RUNX1, WT1

· Hematopoiesis and osteogenesis:EGR3, ESR1, GATA1/2/3, GLI2, NFATC1, PAX9, RB1, SOX6, SP1, STAT1

· Neural stem cell maintenance and differentiation:ATF2, CREB1, FOSB, FOXO3, HES1, HOXD10, MCM2/7, MEF2C, NEUROD1/G1, OLIG1/2, PAX3/6, POU4F1, PPARG, SMAD3/4, SNAI1, STAT3

Note:all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人类干细胞转录因子qPCR阵列板布局 *(8 个粗体和斜体控件)

注意:所有基因名称都遵循HGNC的guanfang符号

1

2

3

4

5

6

7

8

9

10

11

12

A

ATF2

FOSB

FOXP3

HES1

ISL1

MAX

NEUROG1

PAX5

PPARG

SMAD4

STAT3

ACTB

B

CDX2

FOXA1

GATA1

HOXA10

JUN

MCM2

NFATC1

PAX6

PRDM16

SMARCA4

TBX5

GAPDH

C

CREB1

FOXA2

GATA2

HOXA11

KLF2

MCM7

NKX2-2

PAX8

PROX1

SNAI1

TCF3

LDHA

D

DNMT3B

FOXC2

GATA3

HOXA9

KLF4

MEF2C

NOTCH2

PAX9

RB1

SOX2

TDGF1

NONO

E

EGR3

FOXD3

GATA6

HOXB3

KLF5

MSX2

NR2F2

PITX2

RUNX1

SOX6

TLX3

PPIH

F

EOMES

FOXO1

GLI1

HOXB5

LIN28B

MYC

OLIG1

PITX3

SMAD1

SOX9

VDR

GDC

G

ESR1

FOXO3

GLI2

HOXC9

LMX1B

NANOG

OLIG2

POU4F1

SMAD2

SP1

WT1

PPC

H

EZH2

FOXP1

HAND1

HOXD10

MAF

NEUROD1

PAX3

POU5F1

SMAD3

STAT1

ZIC1

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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GK013 人类星型胶质细胞转录因子qPCR检测试剂盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017 人血管生成qPCR检测试剂盒,Human Angiogenesis qPCR Array Kit

GK018 人内皮细胞表型异质性qPCR检测试剂盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

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GK020 人B细胞受体信号通路qPCR检测试剂盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

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GK022 人内吞作用qPCR检测试剂盒,Human Endocytosis qPCR Array Kit

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GK029 Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit

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GK079 人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit

GK080 人成骨分化qPCR检测试剂盒,GeneQuery™ Human Osteogenic Differentiation qPCR Array Kit

GK081 人类周细胞生物学qPCR检测试剂盒,Human Pericyte Biology qPCR Array Kit

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GK093 Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094 Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095 人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

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GK126 人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit

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